Key features and details
- Rabbit polyclonal to GSAP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GSAP antibody
See all GSAP primary antibodies
DescriptionRabbit polyclonal to GSAP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment (His-T7-tag) corresponding to Human GSAP aa 616-854. (Expressed in E.coli).
DHFLRHLQGVEKKKIEQMVLDYISKLLDLICHIVETNWRKHNLHSWVLHF NSRGSAAEFAVFHIMTRILEATNSLFLPLPPGFHTLHTILGVQCLPLHNL LHCIDSGVLLLTETAVIRLMKDLDNTEKNEKLKFSIIVRLPPLIGQKICR LWDHPMSSNIISRNHVTRLLQNYKKQPRNSMINKSSFSVEFLPLNYFIEI LTDIESSNQALYPFEGHDNVDAEFVEEAALKHTAMLLGL
Database link: A4D1B5
- WB: Human serum; Recombinant human GSAP protein. IHC-P: Human prostate and prostate cancer tissues.
Previously labelled as PION.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233435 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 98 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionRegulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta-amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the GSAP family.
modificationsThe protein is first synthesized as a holoprotein form of 98 kDa and rapidly processed into the gamma-secretase-activating protein 16 kDa C-terminal form, which constitutes the predominant form.
Cellular localizationGolgi apparatus > trans-Golgi network.
- Information by UniProt
- A530088I07Rik antibody
- DKFZp667B242 antibody
- Gamma-secretase-activating protein 16 kDa C-terminal form antibody
Formalin-fixed, paraffin-embedded human prostate tissue stained for GSAP using ab233435 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate cancer tissue stained for GSAP using ab233435 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-GSAP antibody (ab233435) at 5 µg/ml + Human serum
Predicted band size: 98 kDa
Anti-GSAP antibody (ab233435) at 2 µg/ml + Recombinant human GSAP protein
Predicted band size: 98 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233435 has not yet been referenced specifically in any publications.