Product nameAnti-GSC2 antibody
See all GSC2 primary antibodies
DescriptionRabbit polyclonal to GSC2
Specificityab65032 detects endogenous levels of total GSC2 protein.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from an internal sequence of human GSC2.
- 293, HepG2 and COLO205 cell extracts.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab65032 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab65032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevanceGSC2, also known as Goosecoidlike or GSCL, is a homeodomain-containing gene that resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities and is phenotypically related to DiGeorge syndrome (DGS), both of which are associated with hemizygous 22q11 deletions.
- GSC 2 antibody
- GSCL antibody
- Homeobox protein goosecoid 2 antibody
- Homeobox protein goosecoid like antibody
All lanes : Anti-GSC2 antibody (ab65032) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : HepG2 cell extract
Lane 3 : COLO205 cell extract
Lane 4 : COLO205 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Immunostaining of mouse E9.5 embryo tissue sections. After using a biotinylated secondary, ABC and then DAB was used for color development. Please see abreview for protocol details.
ab65032 has not yet been referenced specifically in any publications.