Recombinant Anti-GSDMA antibody [EPR19858-104] (ab230768)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19858-104] to GSDMA
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GSDMA antibody [EPR19858-104]
See all GSDMA primary antibodies -
Description
Rabbit monoclonal [EPR19858-104] to GSDMA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate; Hela cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate; mouse skin and stomach tissue lysate; human skin and stomach tissue lysate; rat skin tissue lysate. IP: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. IHC-P: Human skin tissue; mouse stomach tissue; rat stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19858-104 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
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IP | (1) |
1/30.
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Notes |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). |
IP
1/30. |
Target
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Relevance
Function: May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. Tissue specificity: Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and mucus-secreting pit cells of the gastric epithelium. Down-regulateded in gastric cancer cells. Similarity: Belongs to the gasdermin family. Domain: Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain. -
Cellular localization
Cytoplasm, perinuclear region -
Database links
- Entrez Gene: 284110 Human
- Entrez Gene: 57911 Mouse
- Entrez Gene: 360619 Rat
- Omim: 611218 Human
- SwissProt: Q96QA5 Human
- SwissProt: Q9EST1 Mouse
- Unigene: 448873 Human
- Unigene: 458085 Mouse
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Alternative names
- FKSG9 antibody
- Gasdermin 1 antibody
- Gasdermin A antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of rat stomach (PMID: 23979942) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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All lanes : Anti-GSDMA antibody [EPR19858-104] (ab230768) at 1/1000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse stomach tissue lysate
Lane 3 : Human skin tissue lysate
Lane 4 : Human stomach tissue lysate
Lane 5 : Rat skin tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 32 seconds.
Lanes 2 & 3: 3 minutes.
Lanes 4 & 5: 102 seconds.
This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.
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Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of mouse stomach (PMID: 23979942) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of human skin (PMID: 23979942) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab230768 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230768 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).
Lane 2: ab230768 IP in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230768 in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: less than 1 second.The observed molecular mass is consistent with the literature (PMID:17350798).
The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
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All lanes : Anti-GSDMA antibody [EPR19858-104] (ab230768) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Hela cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : Hela cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-GSDMA antibody [EPR19858-104] (ab230768) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : Hela cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
The observed molecular mass is consistent with the literature (PMID:17350798).
The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab230768 has been referenced in 3 publications.
- Fu L et al. CircularRNA circ_0071269 knockdown protects against from diabetic cardiomyopathy injury by microRNA-145/gasdermin A axis. Bioengineered 13:2398-2411 (2022). PubMed: 35034587
- Chen YR et al. siRNAs Targeting Mouse-Specific lncRNA AA388235 Induce Human Tumor Cell Pyroptosis/Apoptosis. Front Oncol 11:662444 (2021). PubMed: 34195074
- Crowley L et al. A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors. Elife 9:N/A (2020). PubMed: 32915138