Recombinant
RabMAb

Recombinant Anti-GSDMA antibody [EPR19858-17] (ab214818)

Overview

  • Product name

    Anti-GSDMA antibody [EPR19858-17]
    See all GSDMA primary antibodies
  • Description

    Rabbit monoclonal [EPR19858-17] to GSDMA
  • Host species

    Rabbit
  • Specificity

    ab214818 also reacts with mouse GSDMA2 and GSDMA3.

    Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.

  • Tested applications

    Suitable for: IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human GSDMA aa 1 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: Hela cells stably transfected with human or mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. Human stomach lysate. Human and rat skin lysate. IHC-Fr: Mouse stomach and breast skin tissue. IP: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19858-17
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.

We recommend to optimize primary antibody dilution depending on the tissue being tested.

WB 1/1000. Predicted molecular weight: 49 kDa.

Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.

IP 1/30.

Target

  • Relevance

    Function: May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. Tissue specificity: Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and mucus-secreting pit cells of the gastric epithelium. Down-regulateded in gastric cancer cells. Similarity: Belongs to the gasdermin family. Domain: Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
  • Cellular localization

    Cytoplasm, perinuclear region
  • Database links

  • Alternative names

    • FKSG9 antibody
    • Gasdermin 1 antibody
    • Gasdermin A antibody
    • Gasdermin-1 antibody
    • Gasdermin-A antibody
    • GSDM antibody
    • GSDM1 antibody
    • GSDMA antibody
    • GSDMA_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMA with ab214818 at 1/300 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in cells localized in the differentiated region of stomach epithelia of mouse stomach tissue section (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
    Antigen retrieval is not needed.

  • All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

    Lane 1 : HeLa cells, whole cell lysate
    Lane 2 : HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate lysate
    Lane 3 : HeLa cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5%NFDM/TBS.

    The observed molecular mass is consistent with the literature (PMID:17350798).

    The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

  • GSDMA was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab214818 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214818 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
    Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 µg (Input).
    Lane 2: ab209847 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214818 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: Less than 1 second.

    The observed molecular mass is consistent with the literature (PMID:17350798).

    The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

  • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse breast skin tissue labeling GSDMA with ab214818 at 1/50 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in skin keratinocytes and hair follicle cells on mouse breast skin, negative staining in basal and stromal cells (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
    Antigen retrieval is not needed.

  • All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human skin lysate
    Lane 3 : Rat skin lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa



    Blocking/Dilution buffer: 5%NFDM/TBST.

    Exposure times:

    Lanes 1/3: 102 seconds

    Lane 2: 3 minutes

    This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.

  • All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate
    Lane 3 : HeLa cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 49 kDa


    Exposure time: 70 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab214818 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Hela GSDMA-stable)
Total protein in input
5000 µg
Immuno-precipitation step
Protein A
Specification
Hela GSDMA-stable

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Verified customer

Submitted Jul 01 2019

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