Recombinant Anti-GSDMA antibody [EPR19858-17] (ab214818)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19858-17] to GSDMA
- Suitable for: IHC-Fr, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GSDMA antibody [EPR19858-17]
See all GSDMA primary antibodies -
Description
Rabbit monoclonal [EPR19858-17] to GSDMA -
Host species
Rabbit -
Specificity
This antibody also reacts with mouse GSDMA2 and GSDMA3.Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.
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Tested applications
Suitable for: IHC-Fr, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hela cells stably transfected with human or mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. Human stomach lysate. Human and rat skin lysate. IHC-Fr: Mouse stomach and breast skin tissue. IP: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19858-17 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/50.
We recommend to optimize primary antibody dilution depending on the tissue being tested. |
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WB |
1/1000. Predicted molecular weight: 49 kDa.
Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB. |
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IP | (1) |
1/30.
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Notes |
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IHC-Fr
1/50. We recommend to optimize primary antibody dilution depending on the tissue being tested. |
WB
1/1000. Predicted molecular weight: 49 kDa. Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB. |
IP
1/30. |
Target
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Relevance
Function: May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. Tissue specificity: Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and mucus-secreting pit cells of the gastric epithelium. Down-regulateded in gastric cancer cells. Similarity: Belongs to the gasdermin family. Domain: Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain. -
Cellular localization
Cytoplasm, perinuclear region -
Database links
- Entrez Gene: 284110 Human
- Entrez Gene: 57911 Mouse
- Entrez Gene: 360619 Rat
- Omim: 611218 Human
- SwissProt: Q96QA5 Human
- SwissProt: Q9EST1 Mouse
- Unigene: 448873 Human
- Unigene: 458085 Mouse
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Alternative names
- FKSG9 antibody
- Gasdermin 1 antibody
- Gasdermin A antibody
see all
Images
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMA with ab214818 at 1/300 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in cells localized in the differentiated region of stomach epithelia of mouse stomach tissue section (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
Antigen retrieval is not needed. -
All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution
Lane 1 : HeLa cells, whole cell lysate
Lane 2 : HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate lysate
Lane 3 : HeLa cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5%NFDM/TBS.
The observed molecular mass is consistent with the literature (PMID:17350798).
The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
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GSDMA was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab214818 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214818 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 µg (Input).
Lane 2: ab209847 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214818 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: Less than 1 second.The observed molecular mass is consistent with the literature (PMID:17350798).
The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse breast skin tissue labeling GSDMA with ab214818 at 1/50 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in skin keratinocytes and hair follicle cells on mouse breast skin, negative staining in basal and stromal cells (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
Antigen retrieval is not needed. -
All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Human skin lysate
Lane 3 : Rat skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDaBlocking/Dilution buffer: 5%NFDM/TBST.
Exposure times:
Lanes 1/3: 102 seconds
Lane 2: 3 minutes
This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.
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All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : HeLa cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 49 kDa
Exposure time: 70 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab214818 has been referenced in 1 publication.
- Xia B et al. Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2. Cell Discov 9:2 (2023). PubMed: 36609376