Recombinant
RabMAb

Recombinant Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)

Overview

  • Product name

    Anti-GSDMB antibody [EPR20841] - BSA and Azide free
    See all GSDMB primary antibodies
  • Description

    Rabbit monoclonal [EPR20841] to GSDMB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human GSDMB. The exact sequence is proprietary.
    Database link: Q8TAX9

  • Positive control

    • Flow Cyt: A431 cells.
  • General notes

    Ab226483 is the carrier-free version of ab215729. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20841
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    The N-terminal moiety promotes pyroptosis. May be acting by homooligomerizing within the membrane and forming pores (PubMed:27281216). The physiological relevance of this observation is unknown.
  • Tissue specificity

    In the gastrointestinal tract, expressed in proliferating cells, including in the basal cell layer of esophagus and in isthmus/neck of stomach.
  • Sequence similarities

    Belongs to the gasdermin family.
  • Domain

    Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
  • Cellular localization

    Cytoplasm. Cytoplasm, cytosol. Cell membrane. Vesicular localization in the apical region of gastric chief cells and colonic surface mucous cells, and the basal region of neuroendocrine cells.
  • Information by UniProt
  • Database links

  • Form

    There are 5 isoforms produced by alternative splicing.
  • Alternative names

    • 123 antibody
    • 156 antibody
    • Gasdermin B antibody
    • Gasdermin like protein antibody
    • Gasdermin-B antibody
    • Gasdermin-like protein antibody
    • GSDMB antibody
    • GSDMB_HUMAN antibody
    • GSDML antibody
    • PP4052 antibody
    • PRO2521 antibody
    see all

Images

  • GSDMB was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged GSDMB expression vector, whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate 10 µg (Input). 

    Lane 2: ab215729 IP in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).

  • GSDMB was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: A431 whole cell lysate 10 µg (Input). 

    Lane 2: ab215729 IP in A431 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling GSDMB with ab215729 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).

References

ab226483 has not yet been referenced specifically in any publications.

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