Product nameAnti-GSDMD antibody
See all GSDMD primary antibodies
DescriptionRabbit polyclonal to GSDMD
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human GSDMD aa 225-275. The exact sequence is proprietary.
Database link: P57764
- IP: GSDMD IP in Jurkat whole cell lysate.
ab245565 has not performed satisfactorily when used for WB of GSDMD in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of GSDMD.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245565 was affinity purified using an epitope specific to GSDMD immobilized on solid support.
Our Abpromise guarantee covers the use of ab245565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
8 - 15 µl/mg lysate
Sequence similaritiesBelongs to the gasdermin family.
- Information by UniProt
- 1810036L03Rik antibody
- DF 5L antibody
- DF5L antibody
GSDMD was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245565 used for IP at 8 µl per reaction. For WB at a 1/400 dilution.
Lane 1: ab245565 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Chemiluminescence detection: 30 seconds.
ab245565 has not yet been referenced specifically in any publications.