Key features and details
- Rabbit polyclonal to GSDMD - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GSDMD antibody - C-terminal
See all GSDMD primary antibodies
DescriptionRabbit polyclonal to GSDMD - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GSDMD aa 434-484 (C terminal). The exact sequence is proprietary.
Database link: P57764
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228824 was affinity purified using an epitope specific to GSDMD immobilized on solid support.
Our Abpromise guarantee covers the use of ab228824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 52 kDa.|
|IP||Use at an assay dependent concentration.
Use at 20 µl/mg lysate.
Sequence similaritiesBelongs to the gasdermin family.
- Information by UniProt
- 1810036L03Rik antibody
- DF 5L antibody
- DF5L antibody
GSDMD was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228824 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab228824 at 1/100 dilution.
Lane 1: ab228824 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection:Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-GSDMD antibody - C-terminal (ab228824) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutes
Lysates were prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228824 has not yet been referenced specifically in any publications.