Anti-GSDMD antibody - C-terminal (ab228824)

Rabbit polyclonal GSDMD antibody. Validated in WB, IP and tested in Human. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-GSDMD antibody - C-terminal
    See all GSDMD primary antibodies
  • Description

    Rabbit polyclonal to GSDMD - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GSDMD aa 434-484 (C terminal). The exact sequence is proprietary.
    Database link: P57764

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). IP: Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab228824 was affinity purified using an epitope specific to GSDMD immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent concentration.

Use at 20 µl/mg lysate.

Target

  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1810036L03Rik antibody
    • DF 5L antibody
    • DF5L antibody
    • DFNA 5L antibody
    • DFNA5L antibody
    • FKSG 10 antibody
    • FKSG10 antibody
    • FLJ12150 antibody
    • Gasdermin antibody
    • Gasdermin D antibody
    • Gasdermin domain containing 1 antibody
    • Gasdermin domain containing protein 1 antibody
    • Gasdermin domain-containing protein 1 antibody
    • Gasdermin-D antibody
    • GasderminD antibody
    • GSDMD antibody
    • GSDMD_HUMAN antibody
    • GSDMDC 1 antibody
    • GSDMDC1 antibody
    see all

Images

  • GSDMD was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole  cell lysate (1 mg for IP, 20% of IP loaded) with ab228824 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab228824 at 1/100 dilution.

    Lane 1: ab228824 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection:Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-GSDMD antibody - C-terminal (ab228824) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 52 kDa


    Exposure time: 3 minutes


    Lysates were prepared using NETN lysis buffer.

References

ab228824 has not yet been referenced specifically in any publications.

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