Overview

  • Product name

    Anti-GSDMD antibody [EPR19829]
    See all GSDMD primary antibodies
  • Description

    Rabbit monoclonal [EPR19829] to GSDMD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human GSDMD aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P57764

  • Positive control

    • WB: Recombinant Human GSDMD protein (ab164113), HeLa, 293T, PC-3, A549 , SH-SY5Y and Jurkat whole cell lysates.
  • General notes

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19829
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).

Target

  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1810036L03Rik antibody
    • DF 5L antibody
    • DF5L antibody
    • DFNA 5L antibody
    • DFNA5L antibody
    • FKSG 10 antibody
    • FKSG10 antibody
    • FLJ12150 antibody
    • Gasdermin antibody
    • Gasdermin D antibody
    • Gasdermin domain containing 1 antibody
    • Gasdermin domain containing protein 1 antibody
    • Gasdermin domain-containing protein 1 antibody
    • Gasdermin-D antibody
    • GasderminD antibody
    • GSDMD antibody
    • GSDMD_HUMAN antibody
    • GSDMDC 1 antibody
    • GSDMDC1 antibody
    see all

Images

  • All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then incubated for 90 min without treatment whole cell lysate.
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then treated with PrgI and PA (a NAIP-NLRC4 inflammasome trigger) for 90 min whole cell lysate.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 23,53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and diluting buffer was 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

  • All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Human Gasdermin-D knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.

  • All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

References

This product has been referenced in:

  • Tao S  et al. Caspase-1-dependent mechanism mediating the harmful impacts of the quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone on the intestinal cells. J Cell Physiol 234:3621-3633 (2019). Read more (PubMed: 30471106) »
See 1 Publication for this product

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