Product nameAnti-GSDMD antibody [EPR19829] - BSA and Azide free
See all GSDMD primary antibodies
DescriptionRabbit monoclonal [EPR19829] to GSDMD - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human GSDMD aa 1-500. The exact sequence is proprietary.
Database link: P57764
- WB: HeLa whole cell lysate.
Ab232485 is the carrier-free version of ab210070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232485 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).|
Sequence similaritiesBelongs to the gasdermin family.
- Information by UniProt
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All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : GSDMD knockout HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210070).
ab232485 has not yet been referenced specifically in any publications.