Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)

Overview

  • Product name

    Anti-GSDMD antibody [EPR19829] - BSA and Azide free
    See all GSDMD primary antibodies
  • Description

    Rabbit monoclonal [EPR19829] to GSDMD - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human GSDMD aa 1-500. The exact sequence is proprietary.
    Database link: P57764

  • Positive control

    • WB: HeLa whole cell lysate.
  • General notes

    Ab232485 is the carrier-free version of ab210070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19829
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).

Target

  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1810036L03Rik antibody
    • DF 5L antibody
    • DF5L antibody
    • DFNA 5L antibody
    • DFNA5L antibody
    • FKSG 10 antibody
    • FKSG10 antibody
    • FLJ12150 antibody
    • Gasdermin antibody
    • Gasdermin D antibody
    • Gasdermin domain containing 1 antibody
    • Gasdermin domain containing protein 1 antibody
    • Gasdermin domain-containing protein 1 antibody
    • Gasdermin-D antibody
    • GasderminD antibody
    • GSDMD antibody
    • GSDMD_HUMAN antibody
    • GSDMDC 1 antibody
    • GSDMDC1 antibody
    see all

Images

  • All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : GSDMD knockout HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 53 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210070).

References

ab232485 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232485.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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