Overview

  • Product name

    Anti-GSDMD antibody [EPR20859]
    See all GSDMD primary antibodies
  • Description

    Rabbit monoclonal [EPR20859] to GSDMD
  • Host species

    Rabbit
  • Specificity

    This antibody is recommended for mouse only in IHC.

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse GSDMD aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9D8T2

  • Positive control

    • WB: Wild-type mouse liver and lung whole cell lysates; C6, PC-12, RAW 264.7 and NIH/3T3 whole cell lysates; Rat brain and liver lysates. IHC-P: Wild type mouse small intestine tissue. Flow Cyt: RAW 264.7 cells. IP: RAW 264.7 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20859
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab219800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
IP 1/30.
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1810036L03Rik antibody
    • DF 5L antibody
    • DF5L antibody
    • DFNA 5L antibody
    • DFNA5L antibody
    • FKSG 10 antibody
    • FKSG10 antibody
    • FLJ12150 antibody
    • Gasdermin antibody
    • Gasdermin D antibody
    • Gasdermin domain containing 1 antibody
    • Gasdermin domain containing protein 1 antibody
    • Gasdermin domain-containing protein 1 antibody
    • Gasdermin-D antibody
    • GasderminD antibody
    • GSDMD antibody
    • GSDMD_HUMAN antibody
    • GSDMDC 1 antibody
    • GSDMDC1 antibody
    see all

Images

  • All lanes : Anti-GSDMD antibody [EPR20859] (ab219800) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
    Lane 5 : Rat liver lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Exposure time : Lanes 1 and 2:3 minutes; Lanes 3 and 4:48 seconds; Lanes 5 and 6:3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-GSDMD antibody [EPR20859] (ab219800) at 1/1000 dilution

    Lane 1 : Wild-type mouse liver whole cell lysate
    Lane 2 : GSDMD knockout mouse liver whole cell lysate
    Lane 3 : Wild-type mouse lung whole cell lysate
    Lane 4 : GSDMD knockout mouse lung whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Exposure time: Lanes 1 and 2:15 seconds; Lanes 3 and 4:37 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody has strong reactivity with GSDMD with a few weak bands of undermined proteins.

    The lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.

     

  • Immunohistochemical analysis of paraffin-embedded Wild type mouse small intestine (A) and GSDMD KO mouse small intestine (B) tissue labeling GSDMD with ab219800 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on epithelium of wild type mouse small intestine (A), no staining on GSDMD KO mouse small intestine (B) (PMID: 17350798) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    The tissue samples were kindly provided by our collaborator Dr. Feng Shao NIBS.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling GSDMD with ab219800 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • GSDMD was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab219800 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219800 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: RAW 264.7 whole cell lysate 10 µg (Input). 

    Lane 2: ab219800 IP in RAW 264.7 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219800 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

  • Wu M  et al. A PLK1 kinase inhibitor enhances the chemosensitivity of cisplatin by inducing pyroptosis in oesophageal squamous cell carcinoma. EBioMedicine 41:244-255 (2019). Read more (PubMed: 30876762) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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