Key features and details
- Rabbit polyclonal to GSG1L
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GSG1L antibody
DescriptionRabbit polyclonal to GSG1L
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human stomach tissue; RT-4, U-251 MG, liver and tonsil lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesBelongs to the GSG1 family.
- Information by UniProt
- Germ cell-specific gene 1-like protein antibody
- GSG1-like protein antibody
- GSG1L antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoskeleton (microtubules). Recommended concentration of ab121432 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121432 at a 1/75 dilution staining GSG1L in paraffin-embedded Human stomach tissue, by Immunohistochemistry.
All lanes : Anti-GSG1L antibody (ab121432) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121432 has been referenced in 1 publication.
- McGee TP et al. Auxiliary Subunit GSG1L Acts to Suppress Calcium-Permeable AMPA Receptor Function. J Neurosci 35:16171-9 (2015). ICC/IF ; Human . PubMed: 26658868