Overview

  • Product name

  • Description

    Rabbit polyclonal to GSH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GSH2 (N terminal). The exact sequence is proprietary.
    Database link: Q9BZM3

  • Positive control

    • WB: IMR32 whole cell lysate. IP: IMR32 whole cell lysate. ICC/IF: SK-N-SH cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

Images

  • 4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for GSH2 (green) using ab229239 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-GSH2 antibody (ab229239) at 1/500 dilution + IMR32 whole cell lysate/extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 32 kDa



    12% SDS-PAGE

  • GSH2 was immunoprecipitated from IMR32 whole cell lysate with 5μg ab190908. Western blot was performed from the immunoprecipitate using ab229239. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: IgG instead of ab229239 in IMR32 whole cell lysate.

    Lane 2: ab229239 IP in IMR32 whole cell lysate. 

     

References

ab229239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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