RecombinantRabMAb

Recombinant Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)

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Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)
  • Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP933Y] to GSK3 (alpha + beta)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GSK3 (alpha + beta) antibody [EP933Y]
    See all GSK3 (alpha + beta) primary antibodies

  • Description

    Rabbit monoclonal [EP933Y] to GSK3 (alpha + beta)

  • Host species

    Rabbit

  • Specificity

    ab62368 detects both GSK3 alpha and GSK3 beta.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GSK3 (alpha + beta). The exact sequence is proprietary.

  • Positive control

    • 293T cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab62368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 51 kDa (alpha isoform) / 47 kDa (beta isoform).

    Is unsuitable for Flow Cyt, ICC, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

    • Cellular localization

      Cytoplasmic and Nuclear

    • Database links

      see all

    • Alternative names

      • Factor A antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase 3 beta antibody
      • GSK3 alpha antibody
      • GSK3 beta antibody
      • GSK3B antibody
      see all

    Images

    • Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)
      Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)
      Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368) at 1/2000 dilution + 293T cell lysate at 10 µg

      Secondary
      goat anti-rabbit, HRP labeled, at 1/2000 dilution

      Predicted band size: 51,47 kDa

    • Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)
      Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368)

    References (6)

    Publishing research using ab62368? Please let us know so that we can cite the reference in this datasheet.

    ab62368 has been referenced in 6 publications.

    • He Q  et al. Mesenchymal stem cell-derived exosomes exert ameliorative effects in type 2 diabetes by improving hepatic glucose and lipid metabolism via enhancing autophagy. Stem Cell Res Ther 11:223 (2020). PubMed: 32513303
    • Hsiao CH  et al. Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm. Stem Cell Res Ther 10:270 (2019). PubMed: 31445515
    • Liao P  et al. Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis. Elife 6:N/A (2017). WB . PubMed: 28440748
    • Li R  et al. Akt SUMOylation regulates cell proliferation and tumorigenesis. Cancer Res 73:5742-53 (2013). PubMed: 23884910
    • Weigand S  et al. Global Quantitative Phosphoproteome Analysis of Human Tumor Xenografts Treated with a CD44 Antagonist. Cancer Res 72:4329-39 (2012). PubMed: 22777824
    • Sundaresan NR  et al. The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun. Nat Med 18:1643-50 (2012). WB ; Mouse . PubMed: 23086477

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