Anti-GSK3 alpha antibody (ab131344)
Key features and details
- Rabbit polyclonal to GSK3 alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSK3 alpha antibody
See all GSK3 alpha primary antibodies -
Description
Rabbit polyclonal to GSK3 alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, surrounding amino acids 19-23 (
T-S-S-F-A
) of Human GSK3 alpha (NP_063937.2). -
Positive control
- MCF7 cells; 293 cell extracts; Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131344 was purified by affinity chromatography using epitope specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
|
|
IHC-P |
1/50 - 1/100.
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|
ICC/IF |
1/100 - 1/200.
|
Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 2931 Human
- Omim: 606784 Human
- SwissProt: P49840 Human
- Unigene: 466828 Human
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Alternative names
- DKFZp686D0638 antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase-3 alpha antibody
see all
Images
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Anti-GSK3 alpha antibody (ab131344) at 1/500 dilution + 293 cell extracts
Predicted band size: 51 kDa -
Immunofluorescent analysis of methanol-fixed MCF7 cells labelling GSK3 alpha with ab131344 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling GSK3 alpha with ab131344 at 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131344 has been referenced in 1 publication.
- Qian CJ et al. Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells. Oncol Lett 12:3499-3505 (2016). WB ; Human . PubMed: 27900027