Recombinant Anti-GSK3 alpha antibody [EP793Y] (ab40870)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-GSK3 alpha antibody [EP793Y]
See all GSK3 alpha primary antibodies -
Description
Rabbit monoclonal [EP793Y] to GSK3 alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GSK3 alpha aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P49840 -
Positive control
- HeLa cell lysate; human breast carcinoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP793Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab40870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/50 - 1/100. | |
| IP | 1/50. |
Target
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Function
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 2931 Human
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: Q2NL51 Mouse
- SwissProt: P18265 Rat
- Unigene: 466828 Human
see all -
Alternative names
- DKFZp686D0638 antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase-3 alpha antibody
see all
Images
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![Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)](https://www.abcam.com/ps/products/40/ab40870/Images/ab40870-280301-anti-gsk3-alpha-antibody-ep793y-western-blot.jpg)
Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GSK3 alpha knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab40870 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40870 was shown to specifically react with GSK3 alpha in wild-type HAP1 cells. No band was observed when GSK3 alpha knockout samples were examined. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)](https://www.abcam.com/ps/products/40/ab40870/Images/ab40870-54149-anti-gsk3-alpha-antibody-ep793y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)ab4870 at 1/50 dilution, staining GSK3 alpha in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)](https://www.abcam.com/ps/products/40/ab40870/Images/ab40870-54150-anti-gsk3-alpha-antibody-ep793y-western-blot.jpg)
Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)Anti-GSK3 alpha antibody [EP793Y] (ab40870) at 1/5000 dilution + 10ug HeLa cell lysate.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Datasheets and documents
References
This product has been referenced in:
- Duthie A et al. Recruitment, Retainment, and Biomarkers of Response; A Pilot Trial of Lithium in Humans With Mild Cognitive Impairment. Front Mol Neurosci 12:163 (2019). Read more (PubMed: 31316348) »
- Krishnankutty A et al. In vivo regulation of glycogen synthase kinase 3ß activity in neurons and brains. Sci Rep 7:8602 (2017). Read more (PubMed: 28819213) »
