Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GSK3 alpha antibody [EP793Y] (ab40870)

Submit a review Submit a question References (6)

Overview

  • Product name

    Anti-GSK3 alpha antibody [EP793Y]
    See all GSK3 alpha primary antibodies

  • Description

    Rabbit monoclonal [EP793Y] to GSK3 alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GSK3 alpha aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P49840

  • Positive control

    • HeLa cell lysate; human breast carcinoma tissue. ICC/IF: HeLa cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab40870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/50.

Target

Images

  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GSK3 alpha knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab40870 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab40870 was shown to specifically react with GSK3 alpha in wild-type HAP1 cells. No band was observed when GSK3 alpha knockout samples were examined. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha antibody [EP793Y] (ab40870)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling GSK3 alpha with purified ab40870 at 1/200 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)

    ab4870 at 1/50 dilution, staining GSK3 alpha in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Anti-GSK3 alpha antibody [EP793Y] (ab40870) at 1/5000 dilution + 10ug HeLa cell lysate.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

References

This product has been referenced in:

  • Duthie A  et al. Recruitment, Retainment, and Biomarkers of Response; A Pilot Trial of Lithium in Humans With Mild Cognitive Impairment. Front Mol Neurosci 12:163 (2019). Read more (PubMed: 31316348) »
  • Sang B  et al. Ras-AKT signaling represses the phosphorylation of histone H1.5 at threonine 10 via GSK3 to promote the progression of glioma. Artif Cells Nanomed Biotechnol 47:2882-2890 (2019). Read more (PubMed: 31307224) »
See all 6 Publications for this product

Publishing research using ab40870? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab40870.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com