Product nameAnti-GSK3 alpha antibody [EP793Y]
See all GSK3 alpha primary antibodies
DescriptionRabbit monoclonal [EP793Y] to GSK3 alpha
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GSK3 alpha aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P49840
- HeLa cell lysate; human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab40870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- DKFZp686D0638 antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase-3 alpha antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GSK3 alpha knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40870 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40870 was shown to specifically react with GSK3 alpha in wild-type HAP1 cells. No band was observed when GSK3 alpha knockout samples were examined. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ab4870 at 1/50 dilution, staining GSK3 alpha in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-GSK3 alpha antibody [EP793Y] (ab40870) at 1/5000 dilution + 10ug HeLa cell lysate.
Predicted band size: 51 kDa
Observed band size: 51 kDa
This product has been referenced in:
- Duthie A et al. Recruitment, Retainment, and Biomarkers of Response; A Pilot Trial of Lithium in Humans With Mild Cognitive Impairment. Front Mol Neurosci 12:163 (2019). Read more (PubMed: 31316348) »
- Krishnankutty A et al. In vivo regulation of glycogen synthase kinase 3ß activity in neurons and brains. Sci Rep 7:8602 (2017). Read more (PubMed: 28819213) »