Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GSK3 alpha antibody [EP793Y] - BSA and Azide free (ab236006)

Overview

  • Product name

    Anti-GSK3 alpha antibody [EP793Y] - BSA and Azide free
    See all GSK3 alpha primary antibodies
  • Description

    Rabbit monoclonal [EP793Y] to GSK3 alpha - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GSK3 alpha aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate; HeLa and HepG2 whole cell lysates.
  • General notes

    Ab236006 is the carrier-free version of ab40870. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236006 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab4870 at 1/50 dilution, staining GSK3 alpha in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40870).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GSK3 alpha knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab40870 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab40870 was shown to specifically react with GSK3 alpha in wild-type HAP1 cells. No band was observed when GSK3 alpha knockout samples were examined. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40870).

References

ab236006 has not yet been referenced specifically in any publications.

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