Overview

  • Product name

    Anti-GSK3 alpha (phospho S21) antibody
    See all GSK3 alpha primary antibodies
  • Description

    Rabbit polyclonal to GSK3 alpha (phospho S21)
  • Host species

    Rabbit
  • Specificity

    ab131112 detects GSK3 alpha only when phosphorylated at serine 21. It does not detect GSK3 alpha phosphorylated at serine 9.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, surrounding the phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3 alpha.

  • Positive control

    • HeLa cells; 293 cell extracts treated with serum; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab131112) at 1/500 dilution

    Lane 1 : 293 cell extracts, untreated
    Lane 2 : 293 cell extracts, treated with serum

    Predicted band size: 51 kDa



    Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking.
  • Immunoflourescent analysis of methanol-fixed HeLa cells labelling GSK3 alpha (phospho S21) with ab131112 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling GSK3 alpha (phospho S21) with ab131112 at 1/50 dilution. The image on the right is treated with blocking peptide.

References

ab131112 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131112.
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