Key features and details
- Rabbit polyclonal to GSK3 alpha (phospho S21)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GSK3 alpha (phospho S21) antibody
See all GSK3 alpha primary antibodies
DescriptionRabbit polyclonal to GSK3 alpha (phospho S21)
Specificityab131112 detects GSK3 alpha only when phosphorylated at serine 21. It does not detect GSK3 alpha phosphorylated at serine 9.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide conjugated to KLH, surrounding the phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3 alpha.
- HeLa cells; 293 cell extracts treated with serum; Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131112 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab131112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- DKFZp686D0638 antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase-3 alpha antibody
All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab131112) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with serum
Predicted band size: 51 kDa
Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking.
Immunoflourescent analysis of methanol-fixed HeLa cells labelling GSK3 alpha (phospho S21) with ab131112 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling GSK3 alpha (phospho S21) with ab131112 at 1/50 dilution. The image on the right is treated with blocking peptide.
ab131112 has not yet been referenced specifically in any publications.