Anti-GSK3 beta antibody (ab131356)
Key features and details
- Rabbit polyclonal to GSK3 beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSK3 beta antibody
See all GSK3 beta primary antibodies -
Description
Rabbit polyclonal to GSK3 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, surrounding amino acids 7-11 (
TTSFA
) of Human GSK3 beta (NP_001139628.1). -
Positive control
- A2780 and HT29 cell extracts; HeLa cells; Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131356 is purified by affinity chromatography using epitope specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
|
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/200.
|
Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
Tissue specificity
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2932 Human
- Omim: 605004 Human
- SwissProt: P49841 Human
- Unigene: 445733 Human
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Alternative names
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
Images
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All lanes : Anti-GSK3 beta antibody (ab131356) at 1/500 dilution
Lane 1 : A2780 cell extract
Lane 2 : HT29 cell extract
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody (ab131356)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling GSK3 beta with ab131356 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
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Immunofluorescence of methanol-fixed HeLa cells staining GSK3 beta with ab131356 at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab131356 has been referenced in 15 publications.
- Wang Q et al. The Occurrence of Valvular Atrial Fibrillation: Involvement of NGF/TrKA Signaling Pathway. J Invest Surg N/A:1-8 (2020). PubMed: 32781864
- Zhang G et al. MicroRNA-124 represses wound healing by targeting SERP1 and inhibiting the Wnt/ß-catenin pathway. Adv Clin Exp Med 28:711-718 (2019). PubMed: 30740945
- Liu YB et al. Silencing long non-coding RNA MEG3 accelerates tibia fraction healing by regulating the Wnt/ß-catenin signalling pathway. J Cell Mol Med 23:3855-3866 (2019). PubMed: 30955246
- Gu M et al. Spinosin and 6'''-Feruloylspinosin protect the heart against acute myocardial ischemia and reperfusion in rats. Mol Med Rep 20:4253-4261 (2019). PubMed: 31545438
- Huang L et al. Galangin decreases p-tau, Aß42 and ß-secretase levels, and suppresses autophagy in okadaic acid-induced PC12 cells via an Akt/GSK3ß/mTOR signaling-dependent mechanism. Mol Med Rep 19:1767-1774 (2019). PubMed: 30628698