Product nameAnti-GSK3 beta antibody
See all GSK3 beta primary antibodies
DescriptionRabbit polyclonal to GSK3 beta
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- A2780 and HT29 cell extracts; HeLa cells; Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131356 is purified by affinity chromatography using epitope specific peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab131356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
Tissue specificityExpressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
Cellular localizationCytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
- Information by UniProt
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
All lanes : Anti-GSK3 beta antibody (ab131356) at 1/500 dilution
Lane 1 : A2780 cell extract
Lane 2 : HT29 cell extract
Predicted band size: 48 kDa
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling GSK3 beta with ab131356 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
Immunofluorescence of methanol-fixed HeLa cells staining GSK3 beta with ab131356 at a dilution of 1/100.
This product has been referenced in:
- Wang M et al. Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3ß pathway. Int J Mol Med 43:2133-2143 (2019). Read more (PubMed: 30864694) »
- Su D et al. GSK3ß and MCL-1 mediate cardiomyocyte apoptosis in response to high glucose. Histochem Cell Biol 152:217-225 (2019). Read more (PubMed: 31197456) »