Overview

  • Product name

  • Description

    Rabbit polyclonal to GSK3 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GSK3 beta. Corresponding to the sequence near the C-terminus.
    Database link: P49841

  • Positive control

    • ICC/IF: Heat shocked HeLa cells.

Applications

Our Abpromise guarantee covers the use of ab223373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Target

  • Function

    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • Tissue specificity

    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all

Images

  • 2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GSK3 beta (red) using ab223373 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit APC at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours room temperature. Heat Shocked at 42°C for 1 hour.

    (A) DAPI nuclear stain (B) ab223373 (C) Merge.

  • 2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GSK3 beta (yellow) using ab223373 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours room temperature. Heat Shocked at 42°C for 1 hour.

    (A) DAPI nuclear stain (B) ab223373 (C) Merge.

References

ab223373 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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