• Product name
    Anti-GSK3 beta antibody [G8]
    See all GSK3 beta primary antibodies
  • Description
    Mouse monoclonal [G8] to GSK3 beta
  • Host species
  • Specificity
    This antibody is specific for GSK3 beta
  • Tested applications
    Suitable for: WB, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human, Xenopus laevis
  • Immunogen

    E.coli produced recombinant Xenopus laevis GSK3 beta.

  • Epitope
    The exact epitope recognised by this antibody has not been mapped.


Our Abpromise guarantee covers the use of ab2602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent dilution.
Use at a concentration of 5 - 10 µg. The protein does not retain the kinase activity after immunoprecipitation.
ELISA 1/1000.
ICC/IF Use at an assay dependent dilution. PubMed: 19047045


  • Function
    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • Tissue specificity
    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • Cellular localization
    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all


  • ab2602 staining GSK3 beta in primary chondrocytes from the Sox9c/c mice after Cre-adenovirus infection by Immunocytochemistry/ Immunofluorescence.


This product has been referenced in:
  • Wang B  et al. miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/ß-catenin pathway in non-small cell lung cancer. Mol Med Rep 18:2753-2761 (2018). Read more (PubMed: 30015869) »
  • Asam K  et al. Eicosanoyl-5-hydroxytryptamide (EHT) prevents Alzheimer's disease-related cognitive and electrophysiological impairments in mice exposed to elevated concentrations of oligomeric beta-amyloid. PLoS One 12:e0189413 (2017). Mouse . Read more (PubMed: 29253878) »
See all 8 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (A549)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Blocking step
Odyssey as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 02 2012

Western blot
Human Cell lysate - whole cell (293FT)
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Chien Hsin Lee

Verified customer

Submitted Sep 11 2009


Thank you for your enquiry. The source of ab2602 replied to us; "In our hands we see only two bands. The weaker one, we believe is the GSK3-alpha. We have no evidence of its reactivity with GSK2-alpha." I hope this information helps, please do not hesitate to contact us again if you need further advice,

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My sincere apologies for the delay in responding to you, I have just this minute received this information from the source of ab2602: "This monoclonal antibody will work well in immunoprecipitation, however, its affect on the GSK3 kinase activity has not been measured. " The source is currently trying to find out more data and I will forward it to you if there is more to come. I hope this information helps,

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