Overview

  • Product name

    GSK3 beta ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    MCF-7 6 2.6%
    Inter-assay
    Sample n Mean SD CV%
    MCF-7 3 6.3%
  • Sample type

    Cell culture extracts, Cell Lysate
  • Assay type

    Sandwich
  • Sensitivity

    5 µg/ml
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    GSK-3β in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit (ab205710) is designed for the semi-quantitative measurement of SK-3β protein in Human and mouse cells.


    Glycogen synthase kinase-3 (GSK3) was initially identified as an enzyme that regulated glycogen synthesis in response to insulin, through its ability to phosphorylate and inactivate glycogen synthase. GSK3, expressed as 2 closely related and similarly regulated isoforms, GSK3A and GSK3B. GSK3B is now known to be involved in a diverse array of signaling cellular processes, including glycogen synthesis, cellular adhesion, and it has been implicated in Alzheimer’s disease. GSK3B is an important element of the PI3 kinase/Akt signaling pathway, and its kinase activity is down-regulated by Akt-mediated phosphorylation at Ser9.

  • Notes

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab205710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Cell line analysis for GSK-3β from 100 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

Protocols

References

ab205710 has not yet been referenced specifically in any publications.

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