Key features and details
- Rabbit polyclonal to GSK3 beta (phospho S9) + GSK3 alpha (phospho S21)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-GSK3 beta (phospho S9) + GSK3 alpha (phospho S21) antibody
DescriptionRabbit polyclonal to GSK3 beta (phospho S9) + GSK3 alpha (phospho S21)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Cow, Human, Pig, Xenopus laevis, Zebrafish
Synthetic peptide within Human GSK3 beta (N terminal) (phospho S9). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P49841
- WB: 16 hour starved NIH/3T3 whole cell extracts, untreated and treated with PDGF.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab226877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46, 51 kDa.|
Cellular localizationGSK3 beta: Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
All lanes : Anti-GSK3 beta (phospho S9) + GSK3 alpha (phospho S21) antibody (ab226877) at 1/1000 dilution
Lane 1 : 16 hour starved NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 2 : 16 hour starved NIH/3T3 (mouse embryo fibroblast cell line), treated with 5 ng/ml PDGF for 15 minutes, whole cell extract
Lysates/proteins at 18 µg per lane.
Predicted band size: 46, 51 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226877 has not yet been referenced specifically in any publications.