Product nameAnti-GSK3 beta (phospho S9) + GSK3 alpha (phospho S21) antibody
DescriptionRabbit polyclonal to GSK3 beta (phospho S9) + GSK3 alpha (phospho S21)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Cow, Human, Pig, Xenopus laevis, Zebrafish
Synthetic peptide within Human GSK3 beta (N terminal) (phospho S9). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P49841
- WB: 16 hour starved NIH/3T3 whole cell extracts, untreated and treated with PDGF.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab226877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46, 51 kDa.|
Cellular localizationGSK3 beta: Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
All lanes : Anti-GSK3 beta (phospho S9) + GSK3 alpha (phospho S21) antibody (ab226877) at 1/1000 dilution
Lane 1 : 16 hour starved NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 2 : 16 hour starved NIH/3T3 (mouse embryo fibroblast cell line), treated with 5 ng/ml PDGF for 15 minutes, whole cell extract
Lysates/proteins at 18 µg per lane.
Predicted band size: 46, 51 kDa
10% SDS-PAGE gel.
ab226877 has not yet been referenced specifically in any publications.