Overview

  • Product name

    Anti-GSK3 beta (phospho S9) antibody
    See all GSK3 beta primary antibodies
  • Description

    Rabbit polyclonal to GSK3 beta (phospho S9)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide around phosphorylation site of Serine 9 (

    T-T-S(p)-F-A)

    of Human GSK3 beta (NP_001139628.1; UniProt P49841), conjugated to KLH.

  • Positive control

    • 293 cell extract treated with serum; HeLa cells; Human breast carcinoma and lung carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab131097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • Tissue specificity

    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all

Images

  • All lanes : Anti-GSK3 beta (phospho S9) antibody (ab131097) at 1/500 dilution

    Lane 1 : 293 cell extract, untreated
    Lane 2 : 293 cell extract treated with serum

    Predicted band size: 46 kDa

  • Immunohistochemical staining of GSK3 beta (phospho S9) in paraffin embedded Human breast carcinoma tissue, using ab131097 at a 1/50 dilution.
  • Immunofluorescence analysis of methanol fixed HeLa cells staining GSK3 beta (phospho S9) with ab131097 at a dilution of 1/100.
  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GSK3 beta (phospho S9) (red) using ab131097 at 1/100 dilution in ICC/IF.

  • Paraffin-embedded human lung carcinoma tissue stained for GSK3 beta (phospho S9) using ab131097 at 1/50 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Yao K  et al. Melatonin receptor stimulation by agomelatine prevents Aß-induced tau phosphorylation and oxidative damage in PC12 cells. Drug Des Devel Ther 13:387-396 (2019). Read more (PubMed: 30718944) »
  • Dai J  et al. Long non-coding RNA CRNDE regulates cell proliferation, migration, invasion, epithelial-mesenchymal transition and apoptosis in oral squamous cell carcinoma. Oncol Lett 17:3330-3340 (2019). Read more (PubMed: 30867767) »
See all 19 Publications for this product

Customer reviews and Q&As

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Answer

Wie am Telefon besprochen, haben wir nun Ihre Bestellung des ab30619 mit dem ab131097 ersetzt. Die Bestellung hat die Nummer xxx und sollte morgen bei Ihnen ankommen.

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