Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
Key features and details
- Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody -
Description
Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) -
Host species
Rabbit -
Specificity
ab75745 detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216 and GSK3 alpha when phosphorylated at tyrosine 276. This antibody can also cross-react with GSK3 alpha subunit due to the consistent antigen-sequence of "GEPNVSYICSRYY".
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human GSK3 beta (phospho Y216).
Synthetic peptide corresponding to Human GSK3 alpha (phospho Y279).
Database link: P49840 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75745 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/200.
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 47 kDa. This antibody can be used with studies using ab110532.
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IHC-P |
1/50 - 1/100.
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Notes |
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ICC/IF
1/100 - 1/200. |
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. This antibody can be used with studies using ab110532. |
IHC-P
1/50 - 1/100. |
Target
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Cellular localization
GSK3 beta: Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. -
Database links
- Entrez Gene: 2931 Human
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- Omim: 606784 Human
see all
Images
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All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with insulin (40µM, 30min)
Predicted band size: 47 kDa
Observed band size: 47 kDa -
ab75745, at a 1/50 dilution, staining GSK3 alpha/beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
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ab75745 at 1/100 dilution staining GSK3 alpha/beta in human Hela cells by ICC/IF.
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All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution
Lane 1 : 293 cells untreated PMA
Lane 2 : 293 cells treated PMA
Predicted band size: 47 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (153)
ab75745 has been referenced in 153 publications.
- Liu JJ et al. Upregulation of TRPM8 can promote the colon cancer liver metastasis through mediating Akt/GSK-3 signal pathway. Biotechnol Appl Biochem 69:230-239 (2022). PubMed: 33432591
- Kim KI et al. Sorafenib induces pigmentation via the regulation of β-catenin signalling pathway in melanoma cells. Exp Dermatol 31:57-63 (2022). PubMed: 32391926
- Lee HJ et al. Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke. Neural Regen Res 17:1556-1565 (2022). PubMed: 34916441
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Bukovac A et al. Bilateral Meningioma: A Case Report and Review of the Literature. Int J Mol Sci 23:N/A (2022). PubMed: 35163107