Question (57782) | GST 6XHis-tag ELISA Kit (ab128573)

Thank you for contacting Abcam earlier today.

I think I have figured out the protocol for this product. Yes, you can use it with a His-tagged protein, but I think the following sentence from page 3 of the protocol guide clarifies the situation:

"Bound proteins can be detected using rabbit antibody specific to the 6XHistagged fusion protein of choice (not included). However this kit does include a detector antibody and standard protein for use with dual GST 6XHis-tagged proteins."

What this means is that if you want to used the kit to detect a protein that is only His tagged, then you also need to use a separate rabbit polyclonal antibody against His aswell (this antibody is NOT supplied with the kit).

I hope that makes sense.

a suitable rabbit polyclonal antibody that can be used in conjunction with the kit would be ab9108:

https://www.abcam.com/6X-His-tag-antibody-ChIP-Grade-ab9108.html



If there is anything else I can help you with then please let me know.