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Could you provide more infromation on how this kit will work with a His-tagged proetin only.
Asked on Aug 14 2012
Thank you for contacting Abcam earlier today.
I think I have figured out the protocol for this product. Yes, you can use it with a His-tagged protein, but I think the following sentence from page 3 of the protocol guide clarifies the situation:
"Bound proteins can be detected using rabbit antibody specific to the 6XHistagged fusion protein of choice (not included). However this kit does include a detector antibody and standard protein for use with dual GST 6XHis-tagged proteins."
What this means is that if you want to used the kit to detect a protein that is only His tagged, then you also need to use a separate rabbit polyclonal antibody against His aswell (this antibody is NOT supplied with the kit).
I hope that makes sense.
a suitable rabbit polyclonal antibody that can be used in conjunction with the kit would be ab9108:
If there is anything else I can help you with then please let me know.
Answered on Aug 14 2012