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]If I uderstand how it works, the anti His Ab is coated on the plate and an anti GST antibody (labeled with HRP, I guess) is used for detection. If my protein only has a His tag, I guess the antibody for detection needs to be an antibody directed against my protein, correct? Is there any other way that one could measure the levels of an His-tagged protein with your kit if an antibody against that protein is not available? Could the antibody for detection be another anti-His antibody?
Asked on Jan 25 2013
You are correct in your understanding of this product. The detector antibody is against GST and your sample will need a GST tag in order to be recognized by this kit. If you were to use another antibody for detection you would want this to be from a rabbit host as the HRP label is against rabbit IgG. I do not think that use of a second His tag would be appropriate with this product as these tags are would be recognized by the capture antibody and would not be presented for the detection antibody. If antibodies to your protein of interest are unavailable we can recommend custom antibody creation by our Epitomics group. More information about these services may be found at: http://www.epitomics.com/services/ or by calling 1-877-772-2622 ext.803
Answered on Jan 25 2013