Anti-GST antibody [3E2] (ab241110)
- Datasheet
- References
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Overview
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Product name
Anti-GST antibody [3E2]
See all GST primary antibodies -
Description
Mouse monoclonal [3E2] to GST -
Host species
Mouse -
Specificity
Detects 26 kDa for purified GST protein. Does not detect proteins without a GST tag. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Fusion protein (GST-tag) corresponding to Schistosoma japonicum GST.
Database link: P08515 -
Positive control
- WB: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein; GST recombinant protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
3E2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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![Western blot - Anti-GST antibody [3E2] (ab241110)](https://www.abcam.com/ps/products/241/ab241110/Images/ab241110-319870-2411101333.jpg)
Western blot - Anti-GST antibody [3E2] (ab241110)All lanes : Anti-GST antibody [3E2] (ab241110) at 1/1000 dilution (2 hours at RT)
Lane 1 : Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein
Lane 2 : GST
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Goat anti-Mouse Ig-HRP for 1 hour at RT at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlock: 5% Skimmed milk powder in TBST.
Datasheets and documents
References
ab241110 has not yet been referenced specifically in any publications.
