Anti-GST antibody [3E2] (ab241110)

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Western blot - Anti-GST antibody [3E2] (ab241110)

    Key features and details

    • Mouse monoclonal [3E2] to GST
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG1

    Overview

    • Product name

      Anti-GST antibody [3E2]
      See all GST primary antibodies

    • Description

      Mouse monoclonal [3E2] to GST

    • Host species

      Mouse

    • Specificity

      Detects 26 kDa for purified GST protein. Does not detect proteins without a GST tag.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Fusion protein (GST-tag) corresponding to Schistosoma japonicum GST.
      Database link: P08515

    • Positive control

      • WB: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein; GST recombinant protein.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab241110 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000.

    Target

    • Relevance

      GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

    • Alternative names

      • Glutathione S Transferase antibody
      • Glutathione S-transferase class-mu 26 kDa isozyme antibody
      • GST antibody
      • Sj26 antigen antibody
      • SjGST antibody
      see all

    Images

    • Western blot - Anti-GST antibody [3E2] (ab241110)
      Western blot - Anti-GST antibody [3E2] (ab241110)
      All lanes : Anti-GST antibody [3E2] (ab241110) at 1/1000 dilution (2 hours at RT)

      Lane 1 : Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein
      Lane 2 : GST

      Lysates/proteins at 1 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse Ig-HRP for 1 hour at RT at 1/5000 dilution

      Developed using the ECL technique.

      Exposure time: 5 minutes


      Block: 5% Skimmed milk powder in TBST.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab241110? Please let us know so that we can cite the reference in this datasheet.

    ab241110 has not yet been referenced specifically in any publications.

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