Overview

  • Product name
    Anti-GST antibody [3G10/1B3]
    See all GST primary antibodies
  • Description
    Mouse monoclonal [3G10/1B3] to GST
  • Host species
    Mouse
  • Specificity
    Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. This is full length GST fusion protein of 425 aa (length of the fusion protein, not GST protein alone). The GST protein is the same GST protein on the pGEX vector ( Schistosoma japonicum).
    Database link: P08515

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP Use at an assay dependent dilution.

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    All lanes : Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution

    Lane 1 : Non-induced GST-fusion protein expressed in E.coli
    Lane 2 : IPTG induced GST-fusion protein expressed in E.coli

    Lysates/proteins at 5 µl per lane.

    Predicted band size: 26 kDa



    10% SDS-PAGE

  • Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    All lanes : Anti-GST antibody [3G10/1B3] (ab92)

    Lane 1 : Total lysate
    Lane 2 : Cytoplasmic fraction
    Lane 3 : Membrane fraction
    Lane 4 : Nuclear fraction

    Predicted band size: 26 kDa



    Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).

References

This product has been referenced in:

See all 14 Publications for this product

Publishing research using ab92? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

8 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-GST antibody [3G10/1B3]

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted May 11 2018

Western blot abreview for Anti-GST antibody [3G10/1B3]

 Excellent
Abreviews
Application
Western blot
Sample
Human Recombinant protein (Recombinant GST protein)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
0.2 µg
Specification
Recombinant GST protein
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Jan 06 2017

Inquiry: Dear Abcam team, I just want to double check that this antibody comes as a powder/lypholyzed sample that I can dilute to the concentration I want. https://www.abcam.com/GST-antibody-3G10-1B3-ab92.html I am inquiring because I require a 0.5mg/m...

Read More

Thank you for your enquiry and your interest in our products.

I can confirm that we sell this antibody in liquid format, the unit size is 100 µg the concentration is 1 mg/ml.

If you need any further assistance in the future, p...

Read More

Hi,   I am wondering what would you recommend me to use as a loading control for human serum sample? Your website only showed what you have available, but I want to know what I can use?  I have been your beta-actin for most of my experiment and it w...

Read More

I am very glad to hear that our anti-Beta Actin loading control has worked so well for you.

In regards to a loading control for your current needs, Beta Actin would only be present in serum if shearing of cells had occurred during collection...

Read More

Western blot abreview for Anti-GST antibody [3G10/1B3]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lysate from Insect Cells Expressing RGS9-GST fusio)
Loading amount
100 µg
Specification
Lysate from Insect Cells Expressing RGS9-GST fusio
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine)
Blocking step
1:1 PBS/Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Read More
abreview image
Username

Dr. Chris Tracy

Verified customer

Submitted Sep 20 2011

BATCH NUMBER 10PX402 ORDER NUMBER 598807 DESCRIPTION OF THE PROBLEM smudged/fuzzy band(s)? SAMPLE Mouse liver samples PRIMARY ANTIBODY Mouse anti-GST (abcam #ab92) was used at a dilution of 1:1000 (3uL in 3mL of PBS/0.05% Twe...

Read More

I'm sorry to hear you are experiencing problems with ab92. I have been able to source the images from the laboratory and have added them to the datasheet, interestingly the band we get with a HeLa whole cell lysate is also large ("fat") in a simila...

Read More

Thanks for your fast reply. I wonder if you would like to reward me another free sample of this product or a catalog of your company? That would be more appreciated.

Thanks again for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should yo...

Read More

on the datasheet, it's said this antibody was raise by targetting 1-425aa of human GST. I didn't find any HUMAN GST had 425aa, normally they were 200aa. I need to know the exact seqence of the antigen which you used to raise this antibody.

Thank you for enquiry. I have checked with the originator of this antibody and there was a mistake regarding the immunogen on the datasheet. This antibody was generated as a bi-product during a fusion. The original immunogen was a GST fusion protei...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up