Anti-GST antibody [3G10/1B3] (ab92)
Key features and details
- Mouse monoclonal [3G10/1B3] to GST
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-GST antibody [3G10/1B3]
See all GST primary antibodies -
Description
Mouse monoclonal [3G10/1B3] to GST -
Host species
Mouse -
Specificity
Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. This is full length GST fusion protein of 425 aa (length of the fusion protein, not GST protein alone). The GST protein is the same GST protein on the pGEX vector ( Schistosoma japonicum).
Database link: P08515 -
General notes
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
w/o preservative -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3G10/1B3 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
|
IP |
Use at an assay dependent dilution.
|
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
IP
Use at an assay dependent dilution. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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All lanes : Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution
Lane 1 : Non-induced GST-fusion protein expressed in E.coli
Lane 2 : IPTG induced GST-fusion protein expressed in E.coli
Lysates/proteins at 5 µl per lane.
Predicted band size: 26 kDa10% SDS-PAGE
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All lanes : Anti-GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate
Lane 2 : Cytoplasmic fraction
Lane 3 : Membrane fraction
Lane 4 : Nuclear fraction
Predicted band size: 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
Protocols
Datasheets and documents
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Datasheet download
References (27)
ab92 has been referenced in 27 publications.
- Lee YH et al. A PIAS1 Protective Variant S510G Delays polyQ Disease Onset by Modifying Protein Homeostasis. Mov Disord 37:767-777 (2022). PubMed: 34951052
- Duan X et al. Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation. Cell Rep 35:109122 (2021). PubMed: 34010635
- Karabiyik C & Rubinsztein DC Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro. STAR Protoc 2:100926 (2021). PubMed: 34766030
- Zhang X et al. PTPN22 interacts with EB1 to regulate T-cell receptor signaling. FASEB J 34:8959-8974 (2020). PubMed: 32469452
- Marquardt J et al. The LKB1-like Kinase Elm1 Controls Septin Hourglass Assembly and Stability by Regulating Filament Pairing. Curr Biol 30:2386-2394.e4 (2020). PubMed: 32386534