Product nameAnti-GST antibody
See all GST primary antibodies
DescriptionGoat polyclonal to GST
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Other species
Full length protein corresponding to Schistosoma japonicum GST aa 2-218.
SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLE FPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLD IRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHP DFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAW PLQGWQATFGGGDHPPK
Database link: P08515
- Recombinant Human GST protein (ab81793) can be used as a positive control in WB. Glutathione-S-Transferase [Schistosoma japonicum]
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181652 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Our Abpromise guarantee covers the use of ab181652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|ELISA||1/200 - 1/1000.
ab181652 has been assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
|IHC-P||Use at an assay dependent concentration.|
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
Lanes 1-4 : Anti-GST antibody (ab181652) at 1/5000 dilution
Lane 5 : Anti-GST antibody (ab181652) at 1/1000 dilution (Reduced)
Lane 6 : Anti-GST antibody (ab181652) at 1/1000 dilution (Non-reduced)
Lanes 1 & 3 & 5-6 : Purified GST at 1 µg
Lanes 2 & 4 : Purified GST at 0.25 µg
ab181652 was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For lanes 1-4, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a Goat anti GST at 1/5000 dilution overnight at 4°C. Primary antibody was detected with a conjugated Donkey anti Goat at 1/10000, incubated for 1.5 hr at RT and imaged on the BioRad VersaDoc imaging system. Lanes 5-6 show a repeat western blot with the same samples (~1 ug per lane, reduced (R) and nonreduced (NR) detected with a Dylight 549 conjugated Donkey anti goat incubated at 1/10000 at 1.5 hours at RT.
ab181652 has not yet been referenced specifically in any publications.