Overview

  • Product name

  • Description

    Rabbit polyclonal to GST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC, ELISAmore details
  • Species reactivity

    Reacts with: Species independent

Properties

Applications

Our Abpromise guarantee covers the use of ab184804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/30000. Predicted molecular weight: 25 kDa.
IP Use a concentration of 1 - 4 µg/ml.
ICC 1/100 - 1/400.

 

 

ELISA 1/1000 - 1/30000.

for coating plates use a dilution of 1:100-1:500

Target

  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Detection of GST-tagged Protein by Western Blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody ab184804 used for WB at 0.06 µg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab184804 has not yet been referenced specifically in any publications.

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