Key features and details
- Rabbit polyclonal to GST
- Suitable for: WB, IP, ICC, ELISA
- Reacts with: Species independent
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab184804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/30000. Predicted molecular weight: 25 kDa.|
|IP||Use a concentration of 1 - 4 µg/ml.|
|ICC||1/100 - 1/400.
|ELISA||1/1000 - 1/30000.
for coating plates use a dilution of 1:100-1:500
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
Detection of GST-tagged Protein by Western Blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody ab184804 used for WB at 0.06 µg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab184804 has not yet been referenced specifically in any publications.