Anti-GST antibody (ab19256)
- Datasheet
- References (34)
- Protocols
Overview
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Product nameAnti-GST antibody
See all GST primary antibodies -
DescriptionRabbit polyclonal to GST
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, ELISA, WB, IP, ChIPmore details
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Species reactivityReacts with: Species independent
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Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab19256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/400. | |
| ELISA | 1/100 - 1/500. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 24 kDa. | |
| IP | Use a concentration of 1 - 4 µg/ml. | |
| ChIP | Use 4 µg for µg of chromatin. |
Target
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RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
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Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Western blot - Anti-GST antibody (ab19256)All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution
Lane 1 : HEK293 lysate at 0.1 µg
Lane 2 : HEK293 lysate at 0.3 µg
Lane 3 : HEK293 lysate at 1 µg
Predicted band size: 24 kDa
Exposure time: 3 secondsDetected by Chemiluminescence.
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Western blot - Anti-GST antibody (ab19256)This image is courtesy of an Abreview submitted by Dr Hanneke OkkenhaugAll lanes : Anti-GST antibody (ab19256) at 1/5000 dilution
Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg
Secondary
All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-GST antibody (ab19256)Image from Joset A et al, J Cell Biol 188:271-85 (2010), Fig 5.NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted
Protocols
References
This product has been referenced in:
- Deng L et al. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation. Cell Res 29:136-150 (2019). Read more (PubMed: 30514904) »
- Zuo Q et al. Interaction of the primordial germ cell-specific protein C2EIP with PTCH2 directs differentiation of embryonic stem cells via HH signaling activation. Cell Death Dis 9:497 (2018). Read more (PubMed: 29703892) »
