Product nameAnti-GST antibody
See all GST primary antibodies
DescriptionRabbit polyclonal to GST
Tested applicationsSuitable for: ICC/IF, ELISA, WB, IP, ChIPmore details
Species reactivityReacts with: Species independent
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab19256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/400.|
|ELISA||1/100 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 24 kDa.|
|IP||Use a concentration of 1 - 4 µg/ml.|
|ChIP||Use 4 µg for µg of chromatin.|
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution
Lane 1 : HEK293 lysate at 0.1 µg
Lane 2 : HEK293 lysate at 0.3 µg
Lane 3 : HEK293 lysate at 1 µg
Predicted band size: 24 kDa
Exposure time: 3 seconds
Detected by Chemiluminescence.
All lanes : Anti-GST antibody (ab19256) at 1/5000 dilution
Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg
All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted
This product has been referenced in:
- Deng L et al. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation. Cell Res 29:136-150 (2019). Read more (PubMed: 30514904) »
- Shang Z et al. LncRNA PCAT1 activates AKT and NF-?B signaling in castration-resistant prostate cancer by regulating the PHLPP/FKBP51/IKKa complex. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30773595) »