Anti-GST antibody (ab19256)
Key features and details
- Rabbit polyclonal to GST
- Suitable for: ICC/IF, ELISA, WB, IP
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GST antibody
See all GST primary antibodies -
Description
Rabbit polyclonal to GST -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ELISA, WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/400.
|
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| ELISA |
1/100 - 1/500.
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| WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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| IP | (1) |
Use a concentration of 1 - 4 µg/ml.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/400. |
|
ELISA
1/100 - 1/500. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
|
IP
Use a concentration of 1 - 4 µg/ml. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Western blot - Anti-GST antibody (ab19256)All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution
Lane 1 : HEK293 lysate at 0.1 µg
Lane 2 : HEK293 lysate at 0.3 µg
Lane 3 : HEK293 lysate at 1 µg
Predicted band size: 24 kDa
Exposure time: 3 secondsDetected by Chemiluminescence.
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Western blot - Anti-GST antibody (ab19256)This image is courtesy of an Abreview submitted by Dr Hanneke OkkenhaugAll lanes : Anti-GST antibody (ab19256) at 1/5000 dilution
Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg
Secondary
All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-GST antibody (ab19256)Image from Joset A et al, J Cell Biol 188:271-85 (2010), Fig 5.NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted
Protocols
References (54)
ab19256 has been referenced in 54 publications.
- Song H et al. HNF4A-AS1/hnRNPU/CTCF axis as a therapeutic target for aerobic glycolysis and neuroblastoma progression. J Hematol Oncol 13:24 (2020). PubMed: 32216806
- Wang P et al. Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7. BMC Plant Biol 19:462 (2019). PubMed: 31675987
- Volpe M et al. Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11. FASEB J 33:9752-9761 (2019). PubMed: 31162950
- Sreeja JS et al. Binding of alpha-fodrin to gamma-tubulin accounts for its role in the inhibition of microtubule nucleation. FEBS Lett 593:1154-1165 (2019). PubMed: 31062342
- Zhong C et al. SREBP2 is upregulated in esophageal squamous cell carcinoma and co-operates with c-Myc to regulate HMGCR expression. Mol Med Rep 20:3003-3010 (2019). PubMed: 31432128
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