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Anti-GST antibody (ab19256)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (54)

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Western blot - Anti-GST antibody (ab19256)
  • Western blot - Anti-GST antibody (ab19256)
  • Immunocytochemistry/ Immunofluorescence - Anti-GST antibody (ab19256)

Key features and details

  • Rabbit polyclonal to GST
  • Suitable for: ICC/IF, ELISA, WB, IP
  • Reacts with: Species independent
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GST antibody [EPR4236] (ab111947)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GST antibody
    See all GST primary antibodies
  • Description

    Rabbit polyclonal to GST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Glutathione-S-Transferase (GST) from Schistosoma japonicum.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GST
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Fusion/marker protein ELISA Kits

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GST protein (ab81793)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab19256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/400.
ELISA
1/100 - 1/500.
WB (4)
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP (1)
Use a concentration of 1 - 4 µg/ml.
Notes
ICC/IF
1/100 - 1/400.
ELISA
1/100 - 1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP
Use a concentration of 1 - 4 µg/ml.

Target

  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Western blot - Anti-GST antibody (ab19256)
    Western blot - Anti-GST antibody (ab19256)
    All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution

    Lane 1 : HEK293 lysate at 0.1 µg
    Lane 2 : HEK293 lysate at 0.3 µg
    Lane 3 : HEK293 lysate at 1 µg

    Predicted band size: 24 kDa


    Exposure time: 3 seconds


    Detected by Chemiluminescence. 

     

  • Western blot - Anti-GST antibody (ab19256)
    Western blot - Anti-GST antibody (ab19256)This image is courtesy of an Abreview submitted by Dr Hanneke Okkenhaug
    All lanes : Anti-GST antibody (ab19256) at 1/5000 dilution

    Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
    Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
    Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg

    Secondary
    All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GST antibody (ab19256)
    Immunocytochemistry/ Immunofluorescence - Anti-GST antibody (ab19256)Image from Joset A et al, J Cell Biol 188:271-85 (2010), Fig 5.

    NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (54)

    Publishing research using ab19256? Please let us know so that we can cite the reference in this datasheet.

    ab19256 has been referenced in 54 publications.

    • Song H  et al. HNF4A-AS1/hnRNPU/CTCF axis as a therapeutic target for aerobic glycolysis and neuroblastoma progression. J Hematol Oncol 13:24 (2020). PubMed: 32216806
    • Wang P  et al. Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7. BMC Plant Biol 19:462 (2019). PubMed: 31675987
    • Volpe M  et al. Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11. FASEB J 33:9752-9761 (2019). PubMed: 31162950
    • Sreeja JS  et al. Binding of alpha-fodrin to gamma-tubulin accounts for its role in the inhibition of microtubule nucleation. FEBS Lett 593:1154-1165 (2019). PubMed: 31062342
    • Zhong C  et al. SREBP2 is upregulated in esophageal squamous cell carcinoma and co-operates with c-Myc to regulate HMGCR expression. Mol Med Rep 20:3003-3010 (2019). PubMed: 31432128
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-GST antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    400 µg
    Immuno-precipitation step
    Other - Dynabeads M-270 Epoxy using Dynabeads Antibody coupling kit (Life Science Technologies)
    Specification
    HeLa cells
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Nov 19 2018

    Western blot abreview for Anti-GST antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% gradient)
    Sample
    Mouse Recombinant protein (made in E.coli)
    Specification
    made in E.coli
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2014

    Question


    Inquiry: 1. Will this antibody recognize GST dimer? 2. I have a GST fused to the c-terminal of my protein expressed in Hela cells. I use 1:1000 dilution. I can see the band expressed from E.coli (using pET3a constructs), but not from Hela (pcDNA5, under CMV promoter). Can you tell me if this antibody recognize that?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 31 2013

    Answer

    Thank you for contacting us.
    The ability for an antibody to recognize an epitope tag can be dependent on the recombinant protein and expression system. We do not have experience using this antibody to detect GST dimer. As shown in the datasheet, this antibody has utility in WB applications (reducing and denaturing conditions) and is able to detect recombinant protein (C-terminal tag) expressed in mammalian cells. At dilutions in the 1:15,000 range and also at higher dilutions (data not shown). Additionally it is able to detect a recombinant GST-tagged protein in as little as 0.1 ug of cell lysate.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 31 2013

    Question

    New lot of ab19256 gives much weaker bands than an older lot.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2011

    Answer

    Thank you for your call today, and I am so sorry that this was not taken care of yesterday. As we discussed, I am sending a free of charge vial of ab19256 on the order ***, which should arrive tomorrow by 10:30am. The FedEx tracking number for this shipment is ***. Please let me know if you have any questions or if there is anything else that we can do for you.  

    Read More

    Abcam Scientific Support

    Answered on Nov 22 2011

    Western blot abreview for Anti-GST antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Purified protein (Recombinant GST protein purified from E. coli)
    Loading amount
    5 µg
    Specification
    Recombinant GST protein purified from E. coli
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Bicine)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 30 2010

    Western blot abreview for Anti-GST antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Recombinant protein (Western blot to analyse GST pulldown)
    Loading amount
    15 µg
    Specification
    Western blot to analyse GST pulldown
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 14 2010

    ChIP abreview for Anti-GST antibody

    Good
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell)
    Specification
    Whole cell
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 30 minute(s) and 0 second(s)
    Specification of the cross-linking agent: High quality formaldehyde
    Detection step
    Semiquantitative PCR
    Read More

    Azharul Islam

    Verified customer

    Submitted Feb 02 2009

    Western blot abreview for Anti-GST antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Purified protein (GST-tagged protein expressed in BL21* cells, fract)
    Loading amount
    0.1 µg
    Specification
    GST-tagged protein expressed in BL21* cells, fract
    Gel Running Conditions
    Reduced Denaturing (8% SDS page gel)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Hanneke Okkenhaug

    Verified customer

    Submitted Nov 05 2008

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