Product nameAnti-GST antibody
See all GST primary antibodies
DescriptionRabbit polyclonal to GST
Tested applicationsSuitable for: ICC/IF, ELISA, WB, IP, ChIPmore details
Species reactivityReacts with: Species independent
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab19256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/400.|
|ELISA||1/100 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 24 kDa.|
|IP||Use a concentration of 1 - 4 µg/ml.|
|ChIP||Use 4 µg for µg of chromatin.|
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution
Lane 1 : HEK293 lysate at 0.1 µg
Lane 2 : HEK293 lysate at 0.3 µg
Lane 3 : HEK293 lysate at 1 µg
Predicted band size: 24 kDa
Exposure time: 3 seconds
Detected by Chemiluminescence.
All lanes : Anti-GST antibody (ab19256) at 1/5000 dilution
Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg
All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted
This product has been referenced in:
- Deng L et al. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation. Cell Res 29:136-150 (2019). Read more (PubMed: 30514904) »
- Zuo Q et al. Interaction of the primordial germ cell-specific protein C2EIP with PTCH2 directs differentiation of embryonic stem cells via HH signaling activation. Cell Death Dis 9:497 (2018). Read more (PubMed: 29703892) »