Overview

  • Product name
  • Description
    Rabbit polyclonal to GST
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IP, ChIPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Glutathione-S-Transferase (GST) from Schistosoma japonicum.

Properties

Applications

Our Abpromise guarantee covers the use of ab19256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/400.
ELISA 1/100 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP Use a concentration of 1 - 4 µg/ml.
ChIP Use 4 µg for µg of chromatin.

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution

    Lane 1 : HEK293 lysate at 0.1 µg
    Lane 2 : HEK293 lysate at 0.3 µg
    Lane 3 : HEK293 lysate at 1 µg

    Predicted band size: 24 kDa


    Exposure time: 3 seconds


    Detected by Chemiluminescence. 

     

  • All lanes : Anti-GST antibody (ab19256) at 1/5000 dilution

    Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
    Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
    Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg

    Secondary
    All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds

    See Abreview

  • NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted

References

This product has been referenced in:
  • Yang Y  et al. Nuclear GSK3ß induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastoma. Int J Oncol 52:709-720 (2018). Read more (PubMed: 29328365) »
  • Siniscalco D  et al. Intraperitoneal Administration of Oxygen/Ozone to Rats Reduces the Pancreatic Damage Induced by Streptozotocin. Biology (Basel) 7:N/A (2018). Read more (PubMed: 29324687) »
See all 27 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abreviews
Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Mouse Recombinant protein (made in E.coli)
Specification
made in E.coli
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 18 2014

Answer

Thank you for contacting us.
The ability for an antibody to recognize an epitope tag can be dependent on the recombinant protein and expression system. We do not have experience using this antibody to detect GST dimer. As shown in the datasheet, this antibody has utility in WB applications (reducing and denaturing conditions) and is able to detect recombinant protein (C-terminal tag) expressed in mammalian cells. At dilutions in the 1:15,000 range and also at higher dilutions (data not shown). Additionally it is able to detect a recombinant GST-tagged protein in as little as 0.1 ug of cell lysate.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question
Answer

Thank you for your call today, and I am so sorry that this was not taken care of yesterday. As we discussed, I am sending a free of charge vial of ab19256 on the order ***, which should arrive tomorrow by 10:30am. The FedEx tracking number for this shipment is ***. Please let me know if you have any questions or if there is anything else that we can do for you.  

Read More
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (Recombinant GST protein purified from E. coli)
Loading amount
5 µg
Specification
Recombinant GST protein purified from E. coli
Gel Running Conditions
Reduced Denaturing (10% Tris-Bicine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Recombinant protein (Western blot to analyse GST pulldown)
Loading amount
15 µg
Specification
Western blot to analyse GST pulldown
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted May 14 2010

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell)
Specification
Whole cell
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: High quality formaldehyde
Detection step
Semiquantitative PCR

Azharul Islam

Verified customer

Submitted Feb 02 2009

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (GST-tagged protein expressed in BL21* cells, fract)
Loading amount
0.1 µg
Specification
GST-tagged protein expressed in BL21* cells, fract
Gel Running Conditions
Reduced Denaturing (8% SDS page gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Hanneke Okkenhaug

Verified customer

Submitted Nov 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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