Overview

  • Product name
  • Description
    Rabbit polyclonal to GST
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with Schistosoma japonicum. Not yet tested on other species.
  • Immunogen

    Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.

Properties

Applications

Our Abpromise guarantee covers the use of ab9085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IP Use at an assay dependent dilution.
WB 1/500.
ICC/IF Use at an assay dependent dilution. PubMed: 21242312

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • ab9085 staining GST in wild-type (GST–NUAK2-wt) expressing HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were treated with 10µM Y27632 inhibitor for 30 minutes before fixation.
  • All lanes : Anti-GST antibody (ab9085) at 1/2000 dilution

    Lane 1 : Recombinant GST protein purified from E. coli XL1-Blue
    Lane 2 : Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 26,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocked with 5% Milk for 1 hour at 25°C.

    See Abreview

References

This product has been referenced in:
  • Malik I  et al. Development of a Cell-Based Assay to Assess Binding of the proNGF Prodomain to Sortilin. Cell Mol Neurobiol 38:827-840 (2018). ICC . Read more (PubMed: 29067536) »
  • McManus FP  et al. Quantitative SUMO proteomics reveals the modulation of several PML nuclear body associated proteins and an anti-senescence function of UBC9. Sci Rep 8:7754 (2018). Read more (PubMed: 29773808) »
See all 44 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (E. coli-purified GST)
Loading amount
0.05 µg
Specification
E. coli-purified GST
Gel Running Conditions
Reduced Denaturing (10% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 21 2013

Answer

Thank you for contacting us.

While it is likely that this product will work in IF, we have not yet tested ab6613 in this application and therefore cannot guarantee that it will give the desired results in this application. We do have a number of anti-GST antibodies which have been tested and are covered by our Abpromise guarantee for use in IF. Two of the better characterized are:

ab9085 https://www.abcam.com/GST-antibody-ab9085.html

ab18183https://www.abcam.com/GST-antibody-GST-B6-ab18183.html



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Our product team has created this form which I have attached. This should be live on the website as well. Please let me know if you have any other questions or if there is anything else Abcam might do for you.

Read More

Answer

Thank you for contacting us.

I have contacted our products team and they are currently creating an MSDS for this product. This should be on the website soon. I will send you a copy when this has been created as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (Recombinant protein purified from E. coli XL1-Blue)
Loading amount
10 µg
Specification
Recombinant protein purified from E. coli XL1-Blue
Gel Running Conditions
Reduced Denaturing (12 % PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 08 2012

Question
Answer

I am sorry for the delay in replying to you. I finally got a confirmation from the originator of the product. According to them, as far as they know, this product will detect all forms of GST. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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