Key features and details
- Rabbit polyclonal to GST (Alkaline Phosphatase)
- Suitable for: WB, ELISA
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Product nameAnti-GST antibody (Alkaline Phosphatase)
See all GST primary antibodies
DescriptionRabbit polyclonal to GST (Alkaline Phosphatase)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with
Recombinant GST from Schistosoma japonicum expressed in E. coli
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.097% Sodium azide
Constituents: 0.0095% Magnesium chloride, 0.605% Tris, 1% BSA
Concentration information loading...
Purification notesWhole antisera is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. The IgG fraction is then conjugated to calf intestinal alkaline phosphatase using glutaraldehyde.
Our Abpromise guarantee covers the use of ab49597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB ELISAApplication notesELISA: 1/400.
WB: 1/2000 when using BCIP/NBT as substrate, 1/20,000 when using a chemiluminescent
substrate. Detects a band of approximately 27.5 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
ab49597 has not yet been referenced specifically in any publications.
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