Key features and details
- Goat polyclonal to GST (Biotin)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-GST antibody (Biotin)
See all GST primary antibodies
DescriptionGoat polyclonal to GST (Biotin)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Species independent
Glutathione-S-Transferase [Schistosoma japonicum]
The antibody was labelled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab71283 was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab71283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 26 kDa.|
|ELISA||1/10000 - 1/50000.|
RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
ab71283 has been referenced in 1 publication.
- Rockel S et al. iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks. Nucleic Acids Res 41:e52 (2013). PubMed: 23258699