Recombinant Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4236] to GST - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-GST antibody [EPR4236] - BSA and Azide free
See all GST primary antibodies -
Description
Rabbit monoclonal [EPR4236] to GST - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
Database link: Q676U5 -
General notes
ab235384 is the carrier-free version of ab111947.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4236 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysateAll lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Blocking and diluting buffer: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).
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GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysateAll lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Blocking and diluting buffer: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab235384 has not yet been referenced specifically in any publications.