Recombinant
RabMAb

Recombinant Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

Overview

  • Product name

    Anti-GST antibody [EPR4236] - BSA and Azide free
    See all GST primary antibodies
  • Description

    Rabbit monoclonal [EPR4236] to GST - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • General notes

    Ab235384 is the carrier-free version of ab111947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235384 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Relevance

      GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
    • Alternative names

      • Glutathione S Transferase antibody
      • Glutathione S-transferase class-mu 26 kDa isozyme antibody
      • GST antibody
      • Sj26 antigen antibody
      • SjGST antibody
      see all

    Images

    • (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab180123, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Secondary: ab131366, Veriblot, 1/10000 dilution
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

    • GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

      Lane 1: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
      Lane 2: Primary: ab111947, 1/1000 dilution
      Sample: 293T transfected with GST tagged protein vector cell lysate
      Lane 3: Primary: ab172730, Rabbit monoclonal IgG
      Sample: 293T transfected with GST tagged protein vector cell lysate

      All lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
      Blocking and diluting buffer: 5% NFDM/TBST

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

    References

    ab235384 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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