Overview

  • Product name
    Anti-GST antibody (HRP)
    See all GST primary antibodies
  • Description
    Rabbit polyclonal to GST (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.

Properties

Applications

Our Abpromise guarantee covers the use of ab3416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/25000.

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

    See Abreview

  • Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

    See Abreview

References

This product has been referenced in:
  • McClurg UL  et al. Human ex vivo prostate tissue model system identifies ING3 as an oncoprotein. Br J Cancer 118:713-726 (2018). Read more (PubMed: 29381681) »
  • Karlsson T  et al. A polybasic motif in ErbB3-binding protein 1 (EBP1) has key functions in nucleolar localization and polyphosphoinositide interaction. Biochem J 473:2033-47 (2016). Read more (PubMed: 27118868) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Abreviews
Application
ELISA
Sample
Anopheles stephensi Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct

Abcam user community

Verified customer

Submitted Dec 08 2014

Application
ELISA
Sample
Aedes aegypti (Mosquito) Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct

Abcam user community

Verified customer

Submitted Dec 08 2014

Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Aedes aegypti (Mosquito) Recombinant protein (Aedes salivary protein)
Specification
Aedes salivary protein
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 26 2014

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1241474.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

PS: The shipment will be direct to you.

Read More

Answer

Thank very much for your patience as it took us more than a month to test this antibody.

The collaborator laboratory have confirmed that they have immunized rabbits with GST isolated from Schistosoma japonicum. The following is their response

"we tested the antibody (by ELISA) versus various 6HIS preps and have found very little, if any reaction to 6HIS. Despite this, we absorbed the antibody versus various 6HIS preps that we have coupled to gels and then retested hoping to see any kind of change. Unfortunately, no change was observed by ELISA...this doesn't mean that there wouldn't be a change in other kinds of testing but for how we can test we are not unable to see a difference before or after absorbing.

They have also confirmed that the antibody does not have any relation to thrombin protein so it should be binding.

I am sorry we are unable to determine what could have gone wrong with this product. Our test show antibody is specific to the target, I guess the way left is trying a different lot. So if you are interested I can send you a new lot free of charge or another antibody against the same target.

I hope this information will be nevertheless helpful. Please do let me know how you would like to proceed further.

Read More

Answer

Thank you for contacting us.

The immunogen sequence does not contain thrombin, it is a GST isolated from Schistosoma japonicum species. The antibody should not be binding to His conjugated proteins.

The band in His-thrombin might be a non specific binding which could be due to, high concentration of antibody used, damaged antibody or wrong storage of antibody. Could you let us know how the antibody was stored? Could you also repeat the experiment to check this further?

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your enquiry. I am pleased to hear the ab3416 antibody has worked well in your experiments.

I can confirm that ab3416 and ab106222 are different antibodies.

I would like to reassure you that our antibodies are covered by our 6 month guarantee in the tested applications and species listed on the individual datasheets.

I am sorry to confirm that as far as we are aware, ab106222 has never been tested in IHC or ICC. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test ab106222 in ICC-IF, IHC-P (paraffin sections) or IHC-Fr (frozen sections), please contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Answer

Thank you for your question and for your patience while I worked to have this antibody added to our catalog. The unconjugated form of ab3416 is now available as ab115044- https://www.abcam.com/GST-antibody-ab115044.html Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Answer

Sorry for the late response as the information you requested needed some double checking. I can confirm that the antibody preparation used for the FITC (ab3445) and HRP (ab3416) GST antibody is the same. I hope this confirmation will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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