Overview

  • Product name

    Anti-GST antibody (HRP)
    See all GST primary antibodies
  • Description

    Goat polyclonal to GST (HRP)
  • Host species

    Goat
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IP, ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Glutathione-S-Transferase [Schistosoma japonicum]

Properties

Applications

Our Abpromise guarantee covers the use of ab58626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 24623412

Works even though HRP conjugated.

ELISA 1/20000.
WB 1/2000 - 1/5000.
IHC-P 1/500 - 1/2000.

Target

  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • ab58626 staining GST in by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

    See Abreview

References

This product has been referenced in:

  • Gomez A  et al. Characterization of TCDD-inducible poly-ADP-ribose polymerase (TIPARP/ARTD14) catalytic activity. Biochem J 475:3827-3846 (2018). Read more (PubMed: 30373764) »
  • Kaundal A  et al. GENERAL CONTROL NONREPRESSIBLE4 Degrades 14-3-3 and the RIN4 Complex to Regulate Stomatal Aperture with Implications on Nonhost Disease Resistance and Drought Tolerance. Plant Cell 29:2233-2248 (2017). Read more (PubMed: 28855332) »
See all 15 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (tumor fibroblast)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
tumor fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Oct 09 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (tumor fibroblast)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
tumor fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Oct 09 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
HeLa lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 01 2019

Abreviews
Application
ELISA
Sample
Rat Purified protein (smooth muscle)
Specification
smooth muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect

Abcam user community

Verified customer

Submitted May 06 2009

Application
Western blot
Sample
Escherichia coli Recombinant protein (Expressed in E.Coli)
Loading amount
10 µg
Specification
Expressed in E.Coli
Gel Running Conditions
Reduced Denaturing (PVDF)
Blocking step
Block-it PBS-T as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 27 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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