Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 4 mU/well
Product nameGST Assay Kit (Colorimetric)
See all GST kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Assay typeEnzyme activity
Sensitivity< 4 mU/well
Assay time1h 30m
Species reactivityReacts with: Mammals, Other species
GST Assay Kit (Colorimetric) (ab65326) is used to detect GST activity in crude cell lysates and purified protein fractions, and also to quantitate GST-tagged fusion proteins.
The GST assay protocol is based on the GST-catalyzed reaction between GSH and the GST substrate CDNB.
CDNB has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm.
One unit of GST activity is defined as the amount of enzyme producing 1 µmol of GS-DNB conjugate/min under the conditions of the assay. Detection limit of assay: active GST < 1 mU.
GST assay protocol summary:
- add samples and positive control to wells
- add glutathione to wells
- add reaction mix
- analyze with a microplate reader for at least 10 min
Glutathione S-transferase (GST, EC 188.8.131.52) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Glutathione (GSH, lyophilized) Yellow 2 x 17mg GST Assay Buffer WM 1 x 25ml GST Positive Control (0.625 µg/µl) Green 1 x 10µl GST Substrate (CDNB) Red 1 x 0.1ml
RelevanceGlutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta.
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
ab65326 has been referenced in 7 publications.
- Almatroodi SA et al. 6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Pharmaceutics 13:N/A (2021). PubMed: 33670981
- Wang Y et al. P66Shc Deletion Ameliorates Oxidative Stress and Cardiac Dysfunction in Pressure Overload-Induced Heart Failure. J Card Fail 26:243-253 (2020). PubMed: 31536807
- Younis NS et al. Protective Effect of Geraniol on Oxidative, Inflammatory and Apoptotic Alterations in Isoproterenol-Induced Cardiotoxicity: Role of the Keap1/Nrf2/HO-1 and PI3K/Akt/mTOR Pathways. Antioxidants (Basel) 9:N/A (2020). PubMed: 33053761
- Bandyopadhaya A et al. Pseudomonas aeruginosa Quorum Sensing Molecule Alters Skeletal Muscle Protein Homeostasis by Perturbing the Antioxidant Defense System. mBio 10:N/A (2019). PubMed: 31575771
- Hang W et al. Piperlongumine and p53-reactivator APR-246 selectively induce cell death in HNSCC by targeting GSTP1. Oncogene 37:3384-3398 (2018). PubMed: 29348462
- Sommer F et al. Site-specific programming of the host epithelial transcriptome by the gut microbiota. Genome Biol 16:62 (2015). Functional Studies ; Mouse . PubMed: 25887251
- Anathy V et al. Oxidative processing of latent Fas in the endoplasmic reticulum controls the strength of apoptosis. Mol Cell Biol 32:3464-78 (2012). PubMed: 22751926