GST Assay Kit (Colorimetric) (ab65326)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 4 mU/well
Overview
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Product name
GST Assay Kit (Colorimetric)
See all GST kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Sensitivity
< 4 mU/well -
Assay time
1h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
GST Assay Kit (Colorimetric) (ab65326) is used to detect GST activity in crude cell lysates and purified protein fractions, and also to quantitate GST-tagged fusion proteins.
The GST assay protocol is based on the GST-catalyzed reaction between GSH and the GST substrate CDNB.
CDNB has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm.
One unit of GST activity is defined as the amount of enzyme producing 1 µmol of GS-DNB conjugate/min under the conditions of the assay. Detection limit of assay: active GST < 1 mU.
GST assay protocol summary:
- add samples and positive control to wells
- add glutathione to wells
- add reaction mix
- analyze with a microplate reader for at least 10 min -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K263 GST Colorimetric Activity Assay Kit. K263-100 is the same size as the 100 test size of ab65326.
Glutathione S-transferase (GST, EC 2.5.1.13) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Reducing Agent I 2 x 17mg GST Assay Buffer 1 x 25ml GST Positive Control 1 x 10µl GST Substrate 1 x 0.1ml -
Research areas
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Relevance
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
Cellular localization
Cytoplasmic -
Alternative names
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
see all
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab65326 has been referenced in 11 publications.
- Almatroodi SA et al. 6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Pharmaceutics 13:N/A (2021). PubMed: 33670981
- Saeed K et al. 17ß-Estradiol Abrogates Oxidative Stress and Neuroinflammation after Cortical Stab Wound Injury. Antioxidants (Basel) 10:N/A (2021). PubMed: 34829553
- Wang Y et al. P66Shc Deletion Ameliorates Oxidative Stress and Cardiac Dysfunction in Pressure Overload-Induced Heart Failure. J Card Fail 26:243-253 (2020). PubMed: 31536807
- Younis NS et al. Protective Effect of Geraniol on Oxidative, Inflammatory and Apoptotic Alterations in Isoproterenol-Induced Cardiotoxicity: Role of the Keap1/Nrf2/HO-1 and PI3K/Akt/mTOR Pathways. Antioxidants (Basel) 9:N/A (2020). PubMed: 33053761
- Bandyopadhaya A et al. Pseudomonas aeruginosa Quorum Sensing Molecule Alters Skeletal Muscle Protein Homeostasis by Perturbing the Antioxidant Defense System. mBio 10:N/A (2019). PubMed: 31575771