• Product name

    GST tag ELISA Kit
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Overall 8.5%
    Sample n Mean SD CV%
    Overall 4%
  • Sample type

    Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s Gluthathione S-Transferase (GST) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of GST tagged protein expression in cell extracts or after purification.

    The assay employs a GST specific antibody coated onto a well plate strips. Standards and samples are pipetted into the wells and GST present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti- GST detector antibody is added. After washing away unbound detector antibody an HRP-conjugated secondary detector antibody (HRP Label) specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GST bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    Typical working ranges

    GST (pure protein) 1 – 200 ng/mL

    GST fusion protein 6 – 400ng/mL


    Gluthathione S-transferase (GST) is a 26 kDa protein typically used as a fusion partner of recombinant proteins to increase the level of protein expression in bacterial systems, enhance solubility, protect from proteolysis, improve folding and allow purification by affinity chromatography on gluthathione resin.

    This GST ELISA Kit is an enzyme immunoassay developed for the sensitive detection and quantitation of GST or GST fusion proteins in cells samples or purified tagged proteins. The quantity of GST can be determined in lysates or intermediate purification fractions by comparing their absorbance with that of a known recombinant GST standard curve. The kit has a detection sensitivity limit of 1 ng/mL of GST. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GST fusion proteins.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform




Our Abpromise guarantee covers the use of ab126581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.


  • All lanes : Anti-GST antibody (ab19256)

    Lane 1 : GST recombinant protein (ab89494)
    Lane 2 : p53 tagged to GST protein (ab43615)

    Validation by western blot. Detector antibody from this kit was tested by western blot.
  • All lanes : GST tag ELISA Kit (ab126581)

    Lane 1 : GST recombinant protein (ab89494)
    Lane 2 : p53 tagged to GST protein (ab43615)

    Validation by western blot. Capture antibody from this kit was tested by western blot.
  • Dynamic Range of a recombinant protein tagged to GST. Recombinant full length protein (Human) p53 with N terminal GST tag (ab43615) was tested in a dilution series from 400 ng/mL to 12 ng/mL using the GST ELISA kit. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope).
  • Example standard curve. GST standard protein was serially diluted from 400 ng/mL to 6 ng/mL. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope). Sensitivity was calculated mathematically to be as low as 1 ng/mL.



ab126581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Gracias por contactarnos de nuevo.

Este kit ha sido diseñado para la medida cuantitativa del tag GST. Lamentablemente no se ha testado en muestras de suero.

Siento no poder ser de más ayuda, si hay algo mas en lo que pueda ayudar, estaré encantada de hacerlo.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this kit did not contain all items as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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