Product nameGST-Tag Protein Expression Check Kit
GST-Tag Protein Expression Check Kit (ab270052) is formulated as a qualitative sandwich lateral flow assay. The conjugate pad contains a mixture of anti-GST antibody conjugated to either 40nm gold nanoparticles or peptide whilst the “Test line” (T line) on the nitrocellulose contains an antibody which binds the peptide. As such, a strong T line will appear in the presence of GST-tagged proteins whereas in the absence of GST-tagged protein the T line will not be visible or will appear as a faint line.
The conjugate pad of the GST-Tag Protein Expression Check strips also contains biotin-gold, which binds streptavidin on the “Control Line” (C line) of the nitrocellulose strips. Hence, the C line is assay-independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.
This product is manufactured by Expedeon, an Abcam company. It was previously called GST-Tag Check&Go! kit. Expedeon product code 4005-0030 is the same as the 30 test size of this product.
Storage instructionsPlease refer to protocols.
Components 30 tests 10X Running Buffer 2 vials GST-Tag Protein Expression Check Strips 30 tests GST-tagged protein (lyophilised) 1 vial
ab270052 has not yet been referenced specifically in any publications.