Overview

  • Product name
    Anti-GST3 / GST pi antibody
    See all GST3 / GST pi primary antibodies
  • Description
    Goat polyclonal to GST3 / GST pi
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    LADQGQSWKEEV

    , corresponding to Internal sequence amino acids 21-32 of Human GST3

  • Positive control
    • Human kidney lysates. Lysate prepared from transfected HEK293 cells transiently expressing GST3 / GST pi.

Properties

Applications

Our Abpromise guarantee covers the use of ab36149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

Target

Images

  • Anti-GST3 / GST pi antibody (ab36149) at 0.01 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-GST3 / GST pi antibody (ab36149) at 0.03 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing GST3 / GST pi

    Predicted band size: 23 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Cooper TK  et al. Use of urinary biomarkers of renal ischemia in a lamb preclinical left ventricular assist device model. Artif Organs 36:820-4 (2012). Sheep . Read more (PubMed: 22428807) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Eye retinal protein)
Loading amount
50 µg
Specification
Eye retinal protein
Gel Running Conditions
Reduced Denaturing (4-14%)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 30 2011

Question
Answer

DISCOUNT CODE: *** Expiration date: April 21st, 2012 I am very pleased to hear you would like to accept our offer and test ab36149 in IHC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry. I am sorry I did not mention this earlier but I can offer you a discount of 15% off the order of ab4606, normal mouse kidney slides: https://www.abcam.com/Kidney-mouse-normal-tissue-slides-ab4606.html Include this discount code with your order of ab4606 to get 15% off: Please note this discount code is good for a single purchase of ab4606 only and must be used  by December 28 2011. Please feel free to contact me if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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