Recombinant Anti-GST3 / GST pi antibody [EPR8262] (ab134934)
![Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)](https://www.abcam.com/ps/products/134/ab134934/Images/ab134934-283285-anti-gst3-gst-pi-antibody-epr8262-western-blot.jpg "Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8262] to GST3 / GST pi
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-GST3 / GST pi antibody [EPR8262]
See all GST3 / GST pi primary antibodies -
Description
Rabbit monoclonal [EPR8262] to GST3 / GST pi -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human GST3/ GST pi aa 50-150. The exact sequence is proprietary.
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Positive control
- K562, Jurkat and Hek 293 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8262 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 2950 Human
- Entrez Gene: 14870 Mouse
- Entrez Gene: 24426 Rat
- Omim: 134660 Human
- SwissProt: P09211 Human
- SwissProt: P19157 Mouse
- SwissProt: P04906 Rat
- Unigene: 523836 Human
see all -
Alternative names
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
Images
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Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab134934 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab134934 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab134934 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)](https://www.abcam.com/ps/products/134/ab134934/Images/ab134934-149119-anti-gst3-gst-pi-antibody-epr8262-western-blot.jpg)
Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)All lanes : Anti-GST3 / GST pi antibody [EPR8262] (ab134934) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa -
![Anti-GST3 / GST pi antibody [EPR8262] (ab134934)](https://www.abcam.com/ps/products/134/ab134934/Images/ab134934-3-benefits-of-recombinant-antibodies.png)
Anti-GST3 / GST pi antibody [EPR8262] (ab134934)
Datasheets and documents
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Datasheet download
References (1)
ab134934 has been referenced in 1 publication.
- Shan G et al. Increase in Blood Glutathione and Erythrocyte Proteins Related to Glutathione Generation, Reduction and Utilization in African-American Old Women with Diabetes. J Sci Technol Environ 5:N/A (0). PubMed: 26770888
