Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GST3 / GST pi antibody [EPR8262] (ab134934)

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Overview

  • Product name

    Anti-GST3 / GST pi antibody [EPR8262]
    See all GST3 / GST pi primary antibodies

  • Description

    Rabbit monoclonal [EPR8262] to GST3 / GST pi

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GST3/ GST pi aa 50-150. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat and Hek 293 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab134934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    • Sequence similarities

      Belongs to the GST superfamily. Pi family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Deafness antibody
      • Deafness X-linked 7 antibody
      • DFN7 antibody
      • FAEES3 antibody
      • Fatty Acid Ethyl Ester Synthase III antibody
      • Glutathione S Transferase 3 antibody
      • Glutathione S Transferase Pi antibody
      • Glutathione S-transferase P antibody
      • Glutathione S-transferase pi 1 antibody
      • GST class-pi antibody
      • GST3 antibody
      • GSTP antibody
      • Gstp1 antibody
      • GSTP1-1 antibody
      • GSTP1_HUMAN antibody
      • PI antibody
      • X linked 7 antibody
      see all

    Images

    • Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)
      Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: Hek293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab134934 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab134934 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab134934 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

       

    • Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)
      Western blot - Anti-GST3 / GST pi antibody [EPR8262] (ab134934)
      All lanes : Anti-GST3 / GST pi antibody [EPR8262] (ab134934) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 23 kDa

    References

    This product has been referenced in:

    • Shan G  et al. Increase in Blood Glutathione and Erythrocyte Proteins Related to Glutathione Generation, Reduction and Utilization in African-American Old Women with Diabetes. J Sci Technol Environ 5:N/A (0). Read more (PubMed: 26770888) »
    See 1 Publication for this product

    Publishing research using ab134934? Please let us know so that we can cite the reference in this datasheet.

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