Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-GST3 / GST pi antibody [EPR8263] (ab138491)

Submit a review Submit a question References (8)
Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Immunocytochemistry - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
  • Anti-GST3 / GST pi antibody [EPR8263] (ab138491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8263] to GST3 / GST pi
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

Overview

  • Product name

    Anti-GST3 / GST pi antibody [EPR8263]
    See all GST3 / GST pi primary antibodies

  • Description

    Rabbit monoclonal [EPR8263] to GST3 / GST pi

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GST3/ GST pi aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: HAP1, Jurkat, and HEK293 cell lysates; ICC: K562 cells; Flow Cyt (intra): Jurkat; IHC-P: Rat and mouse stomach, and human tonsil tissue sections; WB: K-562 whole cell lysate, mouse and rat brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified please use at 1/250 - 1/500 dilution
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250
Notes
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified please use at 1/250 - 1/500 dilution
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250
Application notes
Is unsuitable for IP.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

  • Sequence similarities

    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Images

  • Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    All lanes : Anti-GST3 / GST pi antibody [EPR8263] (ab138491) at 1/1000 dilution (Purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab138491 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab138491 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

     

  • Immunocytochemistry - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunocytochemistry - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling GST3 / GST pi with Purified ab138491 at 1/50 dilution (1.98 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GST3 / GST pi with Purified ab138491 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
    Anti-GST3 / GST pi antibody [EPR8263] (ab138491)

References (8)

Publishing research using ab138491? Please let us know so that we can cite the reference in this datasheet.

ab138491 has been referenced in 8 publications.

  • Xuan Y  et al. Inhibition of chaperone-mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer. Mol Med Rep 23:N/A (2021). PubMed: 33760140
  • Pang Y  et al. HIGD-1B inhibits hypoxia-induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24:N/A (2021). PubMed: 34080026
  • Wang B  et al. Oridonin overcomes the gemcitabine resistant PANC-1/Gem cells by regulating GST pi and LRP/1 ERK/JNK signalling. Onco Targets Ther 12:5751-5765 (2019). PubMed: 31410021
  • Wang Y  et al. Herba Gelsemii elegantis is detoxified by ramulus et folium Mussaendae pubescentis extract by modulating hepatic cytochrome P450 and glutathione S-transferase enzymes in rats. Exp Ther Med 15:226-234 (2018). PubMed: 29375684
  • Raghunath A  et al. Genome-wide identification and analysis of Nrf2 binding sites - Antioxidant response elements in zebrafish. Toxicol Appl Pharmacol 360:236-248 (2018). PubMed: 30243843
  • He L  et al. Chronic Microcystin-LR Exposure Induces Hepatocarcinogenesis via Increased Gankyrin in Vitro and in Vivo. Cell Physiol Biochem 49:1420-1430 (2018). PubMed: 30205410
  • Sawada S  et al. Proteomic analysis of 3-MCPD and 3-MCPD dipalmitate-induced toxicity in rat kidney. Arch Toxicol 90:1437-48 (2016). PubMed: 26253146
  • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . PubMed: 24469049

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab138491.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com