Product nameAnti-GST3 / GST pi antibody [EPR8263]
See all GST3 / GST pi primary antibodies
DescriptionRabbit monoclonal [EPR8263] to GST3 / GST pi
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GST3/ GST pi aa 100-200. The exact sequence is proprietary.
- K562, PC3, Hek 293, Human cerebellum and Jurkat lysates; Human breast carcinoma tissue;
Storage instructionsShipped at 4°C. Store at -20ºC.
Dissociation constant (KD)KD = 1.00 x 10 -10 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab138491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Sequence similaritiesBelongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
- Information by UniProt
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab138491 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab138491 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling GST3 / GST pi with ab138491 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-GST3 / GST pi antibody [EPR8263] (ab138491) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : PC3 lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Immunofluorescent analysis of K562 cells labelling GST3 / GST pi with ab138491 at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Wang Y et al. Herba Gelsemii elegantis is detoxified by ramulus et folium Mussaendae pubescentis extract by modulating hepatic cytochrome P450 and glutathione S-transferase enzymes in rats. Exp Ther Med 15:226-234 (2018). Read more (PubMed: 29375684) »
- Raghunath A et al. Genome-wide identification and analysis of Nrf2 binding sites - Antioxidant response elements in zebrafish. Toxicol Appl Pharmacol 360:236-248 (2018). Read more (PubMed: 30243843) »