Recombinant Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-GST3 / GST pi antibody [EPR8263]
See all GST3 / GST pi primary antibodies -
Description
Rabbit monoclonal [EPR8263] to GST3 / GST pi -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GST3/ GST pi aa 100-200. The exact sequence is proprietary.
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Positive control
- K562, PC3, Hek 293, Human cerebellum and Jurkat lysates; Human breast carcinoma tissue;
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 1.00 x 10 -10 M Learn more about KD -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8263 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab138491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 2950 Human
- Entrez Gene: 14870 Mouse
- Entrez Gene: 24426 Rat
- Omim: 134660 Human
- SwissProt: P09211 Human
- SwissProt: P19157 Mouse
- SwissProt: P04906 Rat
- Unigene: 523836 Human
see all -
Alternative names
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
Images
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![Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)](https://www.abcam.com/ps/products/138/ab138491/Images/ab138491-283288-anti-gst3-gst-pi-antibody-epr8263-western-blot.jpg)
Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab138491 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab138491 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)](https://www.abcam.com/ps/products/138/ab138491/Images/ab138491-153751-anti-gst3-gst-pi-antibody-epr8263-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling GST3 / GST pi with ab138491 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)](https://www.abcam.com/ps/products/138/ab138491/Images/ab138491-153752-anti-gst3-gst-pi-antibody-epr8263-western-blot.jpg)
Western blot - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)All lanes : Anti-GST3 / GST pi antibody [EPR8263] (ab138491) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : PC3 lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)](https://www.abcam.com/ps/products/138/ab138491/Images/ab138491-153750-anti-gst3-gst-pi-antibody-epr8263-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)Immunofluorescent analysis of K562 cells labelling GST3 / GST pi with ab138491 at 1/100 dilution. -
![Other - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)](https://www.abcam.com/ps/products/138/ab138491/Images/ab138491-153749-anti-gst3-gst-pi-antibody-epr8263.jpg)
Other - Anti-GST3 / GST pi antibody [EPR8263] (ab138491)
Protocols
References
This product has been referenced in:
- Wang Y et al. Herba Gelsemii elegantis is detoxified by ramulus et folium Mussaendae pubescentis extract by modulating hepatic cytochrome P450 and glutathione S-transferase enzymes in rats. Exp Ther Med 15:226-234 (2018). Read more (PubMed: 29375684) »
- Raghunath A et al. Genome-wide identification and analysis of Nrf2 binding sites - Antioxidant response elements in zebrafish. Toxicol Appl Pharmacol 360:236-248 (2018). Read more (PubMed: 30243843) »
