Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

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Overview

  • Product name

    Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free
    See all GST3 / GST pi primary antibodies

  • Description

    Rabbit monoclonal [EPR8263] to GST3 / GST pi - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GST3/ GST pi aa 100-200. The exact sequence is proprietary.

  • Positive control

    • K562, PC3, Hek 293, Human cerebellum and Jurkat lysates; Human breast carcinoma tissue;

  • General notes

    Ab245762 is the carrier-free version of ab138491. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245762 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab245762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    • Sequence similarities

      Belongs to the GST superfamily. Pi family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Deafness antibody
      • Deafness X-linked 7 antibody
      • DFN7 antibody
      • FAEES3 antibody
      • Fatty Acid Ethyl Ester Synthase III antibody
      • Glutathione S Transferase 3 antibody
      • Glutathione S Transferase Pi antibody
      • Glutathione S-transferase P antibody
      • Glutathione S-transferase pi 1 antibody
      • GST class-pi antibody
      • GST3 antibody
      • GSTP antibody
      • Gstp1 antibody
      • GSTP1-1 antibody
      • GSTP1_HUMAN antibody
      • PI antibody
      • X linked 7 antibody
      see all

    Images

    • Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: Hek293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab138491 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab138491 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

       

    • Other - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Other - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

    • Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

      Immunofluorescent analysis of K562 cells labelling GST3 / GST pi with ab138491 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling GST3 / GST pi with ab138491 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab245762 has not yet been referenced specifically in any publications.

    Publishing research using ab245762? Please let us know so that we can cite the reference in this datasheet.

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