Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

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Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Immunocytochemistry - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Flow Cytometry - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
  • Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8263] to GST3 / GST pi - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free
    See all GST3 / GST pi primary antibodies

  • Description

    Rabbit monoclonal [EPR8263] to GST3 / GST pi - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, Jurkat, and HEK293 cell lysates; ICC: K562 cells; Flow Cyt: Jurkat; IHC-P: Rat and mouse stomach, and human tonsil tissue sections; WB: K-562 whole cell lysate, mouse and rat brain lysates.

  • General notes

    Ab245762 is the carrier-free version of ab138491. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245762 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab245762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    • Sequence similarities

      Belongs to the GST superfamily. Pi family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Deafness antibody
      • Deafness X-linked 7 antibody
      • DFN7 antibody
      • FAEES3 antibody
      • Fatty Acid Ethyl Ester Synthase III antibody
      • Glutathione S Transferase 3 antibody
      • Glutathione S Transferase Pi antibody
      • Glutathione S-transferase P antibody
      • Glutathione S-transferase pi 1 antibody
      • GST class-pi antibody
      • GST3 antibody
      • GSTP antibody
      • Gstp1 antibody
      • GSTP1-1 antibody
      • GSTP1_HUMAN antibody
      • PI antibody
      • X linked 7 antibody
      see all

    Images

    • Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      All lanes : Anti-GST3 / GST pi antibody [EPR8263] (ab138491) at 1/1000 dilution (Purified)

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Rat brain lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 23 kDa



      This data was developed using ab138491, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      This data was developed using ab138491, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: GST3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: Hek293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab138491 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Ab138491 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

       

    • Immunocytochemistry - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Immunocytochemistry - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      This data was developed using ab138491, the same antibody clone in a different buffer formulation.
      Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling GST3 / GST pi with Purified ab138491 at 1/50 dilution (1.98 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Flow Cytometry - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      This data was developed using ab138491, the same antibody clone in a different buffer formulation.
      Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GST3 / GST pi with Purified ab138491 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      This data was developed using ab138491, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      This data was developed using ab138491, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

    • Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
      Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab245762? Please let us know so that we can cite the reference in this datasheet.

    ab245762 has not yet been referenced specifically in any publications.

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