Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GST3 / GST pi antibody [EPR8263] (HRP) (ab202930)

Overview

  • Product name

    Anti-GST3 / GST pi antibody [EPR8263] (HRP)
    See all GST3 / GST pi primary antibodies
  • Description

    Rabbit monoclonal [EPR8263] to GST3 / GST pi (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GST3/ GST pi. The exact sequence is proprietary.
    Database link: P09211

  • Positive control

    • WB: K562, PC3 and Jurkat whole cell lysates and human cerebellum tissue lysate. IHC-P: FFPE human placenta (normal) tissue sections.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/625. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Sequence similarities

    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Images

  • All lanes : Anti-GST3 / GST pi antibody [EPR8263] (HRP) (ab202930) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : GSTP1 (GST3 / GST pi) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 10 seconds


    ab202930 was shown to specifically react with GST3 / GST pi in wild-type HAP1 cells as signal was lost in GSTP1 (GST3 / GST pi) knockout cells. Wild-type and GSTP1 (GST3 / GST pi) knockout samples were subjected to SDS-PAGE. Ab202930 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • IHC image of GST3/GST pi staining in a section of formalin-fixed paraffin-embedded normal human placenta tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab202930, 1/625 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-GST3 / GST pi antibody [EPR8263] (HRP) (ab202930) at 1/5000 dilution

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : PC3 (Human Prostate carcinoma) Whole Cell Lysate
    Lane 3 : Human Cerebellum Tissue Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 2 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202930 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab202930 has not yet been referenced specifically in any publications.

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