Overview

  • Product name

    Anti-GST3 / GST pi antibody - N-terminal
    See all GST3 / GST pi primary antibodies
  • Description

    Rabbit polyclonal to GST3 / GST pi - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human GST3/ GST pi (N terminal). (Carrier-protein conjugated).
    Database link: P09211

  • Positive control

    • IHC-P: Human liver tissue. ICC/IF: HeLa cells. WB: A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab230419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

ICC/IF 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.

Target

Images

  • Anti-GST3 / GST pi antibody - N-terminal (ab230419) at 1/5000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS-PAGE gel.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for GST3 / GST pi using ab230419 at 5µg/ml in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GST3 / GST pi (green) using ab230419 at 1/200 dilution in ICC/IF (left), and merged with DNA probe (right).

References

ab230419 has not yet been referenced specifically in any publications.

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